腹腔感染大鼠肠黏膜通透性的改变与E-cadherin蛋白表达的关系  

Correlation between intestinal permeability and expression of E-cadherin protein in intestinal mucosa in rats with intraperitoneal infection

作　　者：成红刚[1] 丁连安[1] 曲林林[1] 牛冬光[1] 

机构地区：[1]青岛大学医学院附属医院普外科,山东省青岛市266061

出　　处：《世界华人消化杂志》2012年第26期2479-2484,共6页World Chinese Journal of Digestology

基　　金：黎介寿院士肠道屏障研究专项研究基金资助项目;No.LJS-2009002;山东省自然科学基金资助项目;No.ZR2010HM124~~

摘　　要：目的:观察腹腔感染大鼠肠屏障损伤后肠通透性的变化,及其与E-cadherin蛋白表达的相关性.方法:健康成年♂Wistar大鼠40只,随机分为空白对照组(仅行单纯剖腹手术)和腹腔感染组(采用盲肠结扎穿孔法制作腹腔感染模型),术后12、24、36、48h取材,每组8只,处死后,距回盲部10cm处取回肠进行常规病理检查,同时测定肠绒毛高度、肠上皮损伤指数及相应时间点血浆D-乳酸含量,应用Western blot方法测定肠黏膜E-cadherin蛋白的水平.结果:腹腔感染12h后肠屏障开始出现明显损伤性改变:肠绒毛高度明显降低,肠黏膜损伤评分增加,血浆D-乳酸含量明显增加(P<0.01);24h损伤达到高峰:肠绒毛高度最低,肠黏膜损伤评分最大,血浆D-乳酸含量明显增高(P<0.05);随后肠屏障逐渐恢复,但48h组损伤程度仍显著高于对照组(P<0.01).与对照组比较,腹腔感染组E-cadherin蛋白表达量降低(P<0.01);实验组各组相比较,24h组E-cadherin蛋白水平最低,36h和48h时E-cadherin蛋白表达量开始降升高,但仍较对照组低(P<0.01).相关性检验发现血浆D-乳酸水平与E-cadherin蛋白含量具有明显负相关(r=-0.605,P<0.01).结论:腹腔感染状态下,肠屏障严重受损,肠黏膜通透性的改变与E-cadherin蛋白水平具有显著的相关性.AIM：To observe the correlation between intestinal permeability and the expression levels of E-cadherin protein in the intestinal mucosa in rats with intraperitoneal infection. METHODS：Forty healthy adult Wistar rats were randomly divided into control group （n = 8） and abdominal infection group （n = 32）. The control group underwent celiotomy only, while the abdominal infection group was further equally divided into four subgroups and underwent cecal ligation and puncture to induce abdominal infection at 12, 24, 36, and 48 h after celiotomy. All the rats were sacrificed, and ileum tissue 10 cm away from the ileocecal junction was sampled for histological examination and evaluation of mucosal damage. Plasma D-lactate was measured at various time points. The expression of E-cadherin protein in the intestinal mucosa was detected using Western blot. RESULTS：Intestinal barrier began to show significant changes 12 h after induction of infection. At 12 h, the abdominal infection group had lower villous thickness, higher mucosal damage index and plasma levels of D-lactat （all P〈0.01） than the control group. The damage reached the peak at 24 h, and lowest villus height, maximum mucosal injury score, and highest lever of D-lactate were observed at this time point （all P〈0.05）. The level of D-lactate began to fall at 36 h but remained higher than that in the control group （P〈0.01）. The expression level of E-cadherin protein was lowest at 24 h （P〈0.05）, and began to rise at 36 and 48 h but remained lower than those in the control group （both P〈0.01）. D-lactate levels had a significant correlation with the expression levels of E-cadherin protein （r = -0.605, P〈0.01）. CONCLUSION：There is a significant correlation between intestinal permeability and the expression levels of E-cadherin protein in the intestinal mucosa in rats with intraperitoneal infection.

关 键 词：腹腔感染 肠屏障 肠通透性 D-乳酸 E-cadherin 

分 类 号：R363[医药卫生—病理学]