外周血中hTERT及MUC4基因的表达在胰腺癌诊断中的意义  

Clinical significance of expression of hTERT and MUC4 mRNAs in peripheral blood of patients with pancreatic cancer

作　　者：孙大伟[1] 何俊闯[2] 邱法波[3] 张晓峰[4] 黄飞龙[1] 

机构地区：[1]青岛大学医学院,山东省青岛市266071 [2]河南宏力医院普外科,河南省长垣县453400 [3]青岛大学医学院附属医院肝胆外科,山东省青岛市266003 [4]中国人民解放军第302医院肝胆外科二中心一科,北京市100039

出　　处：《世界华人消化杂志》2012年第26期2500-2505,共6页World Chinese Journal of Digestology

摘　　要：目的:探讨人端粒酶逆转录酶(human Telomerase reverse transcriptase,hTERT)及黏蛋白4(mucin4,MUC4)基因在胰腺癌患者外周血中的表达及临床意义.方法:采用实时荧光定量PCR方法检测hTERT mRNA和MUC4 mRNA在胰腺癌患者及正常人外周血中的表达,对hTERT mRNA和MUC4 mRNA的检测结果进行评价分析.结果:胰腺癌组外周血中hTERT mRNA,MUC4 mRNA表达水平分别为(7.95±5.46,38.25±25.07)显著高于对照组(0.92±1.07,4.37±5.96).差异具有显著统计学意义(均P<0.05).hTERT mRNA和MUC4 mRNA均与肿瘤的淋巴结转移、周围器官或远处转移及TNM分期相关(hP分别为0.036、0.027、0.019,均P<0.05;MP分别为0.041、0.022、0.017,均P<0.05).结论:外周血中hTERT mRNA和MUC4 mRNA的表达与胰腺癌侵袭及转移密切相关,可作为诊断胰腺癌的标志物.Abstract AIM： To analyze the clinical significance of expression of human telomerase reverse transcriptase （hTERT） and rnucin 4 （MUC4） mRNAs in peripheral blood of patients with pancreatic cancer. METHODS： Real-time fluorescence quantitative RT-PCR was applied to investigate the quantitative expression of hTERT and MUC4 mRNAs in peripheral blood of patients with pancreatic cancer and healthy controls. RESULTS： The expression levels of hTERT and MUC4 mRNAs in peripheral blood of patients with pancreatic cancer were significantly higher than those in normal controls （7.95 ＋ 5.46 vs 0.92 ± 1.07, P 〈 0.01; 38.25 ± 25.07 vs 4.37 ± 5.96, P 〈 0.05）. The expression of both hTERT and MUC4 mRNAs was associated with lymph node metastasis, surrounding organ infiltration, distant metastasis, and TNM stage （hp = 0.036, 0.027, 0.019; Mp = 0.041, 0.022, 0.017）. CONCLUSION： Abnormal expression of hTERT and MUC4 mRNAs closely correlates with inva- sion and metastasis of pancreatic cancer, and hTERT and MUC4 mRNAs in peripheral blood cells might be useful markers for pancreatic caner.

关 键 词：人端粒酶逆转录酶 黏蛋白4 胰腺癌 实时荧光定量PCR 

分 类 号：R735.9[医药卫生—肿瘤]