消化液蛋白质组学在消化系肿瘤诊断中的作用  

Advances in proteomics of digestive juices for the diagnosis of digestive system malignancies

作　　者：万勤思[1] 张焜和[1] 

机构地区：[1]南昌大学第一附属医院消化内科江西省消化疾病研究所,江西省南昌市330006

出　　处：《世界华人消化杂志》2012年第27期2589-2594,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81060197~~

摘　　要：体液蛋白组学分析为疾病诊疗研究提供了新的技术与策略.血液是临床蛋白分析最常用的标本,但其作为一种系统性体液有其不足之处:成分复杂,与疾病相关的蛋白丰度很低,而局部性体液接近病变部位,其蛋白质组学分析能更好地反映疾病相关信息.消化系统产生胃液等多种局部体液,当相关器官发生病变时,相应消化液的蛋白质组成和数量将发生改变,因而消化液被认为是消化疾病标志物库.近年来关于唾液、胃液、胆汁和胰液的蛋白组学研究有不少报道,发现了一些消化系肿瘤相关蛋白,其中有的显示出良好的诊断应用前景,而结果的重复性、标本处理的标准化等是消化液蛋白组学分析需解决的技术问题.Body fuid proteomic analysis is a new technology and strategy for disease diagnosis and treatment.Blood is the most commonly used specimen in body f uid proteomics,but as a systemic f uid,it has limitations because of complex composition and low abundance of disease-related proteins.In contrast,local body f uids are closest to the lesions,contain more pathological information,and therefore are more valuable in clinical proteomics.In the digestive system,there are a variety of body fluids which are considered potential reservoirs of biomarkers for their quality and quantity of proteins will alter during lesions occurring in corresponding organs.In recent years,more and more clinical proteomic analyses of saliva,gastric juice,bile and pancreatic juice has been reported and the proteins related to digestive cancers have been found,and some proteins show application potentialsin cancer diagnosis.However,the proteomic analyses of digestive juices are facing technical challenges in terms of the reproducibility of results and standardization of specimen handling.

关 键 词：消化液 蛋白质组学 消化系统恶性肿瘤 诊断 

分 类 号：R735[医药卫生—肿瘤]