TIMP-3和RASSF1A在大肠癌中的表达及临床意义  

Clinical significance of TIMP-3 and RASSF1A expression in colorectal cancer

作　　者：顾全凯 闫庆辉[2] 王凤安[2] 薛平[2] 

机构地区：[1]唐山市工人医院普外腔镜科,河北省唐山市063000 [2]河北医科大学第二医院中心实验室,河北省石家庄市050000

出　　处：《世界华人消化杂志》2012年第27期2642-2647,共6页World Chinese Journal of Digestology

摘　　要：目的:探讨TIMP-3和RASSF1A在大肠癌发生发展中的作用及与临床病理特征之间的关系,并且研究TIMP-3和RASSF1A之间的相关性.方法:应用免疫组织化学法检测大肠癌组织,大肠癌旁组织及正常大肠黏膜组织中TIMP-3和RASSF1A蛋白的表达量并结合患者的年龄、性别、分化程度、淋巴结转移等临床病理因素进行综合分析.采用Spearman等级相关分析TIMP-3和RASSF1A之间的相关性.结果:在正常大肠黏膜、癌旁和腺癌中TIMP-3的阳性率分别为90.0%、70.0%和16.7%,RASSF1A阳性率分别为83.3%、63.3%和23.3%.大肠癌中TIMP-3和RASSF1A的表达量与淋巴结转移(P<0.05;P<0.01)、浸润深度(P<0.05;P<0.01)及分化程度(P<0.05;P<0.05)有关.运用Spearman等级相关分析,TIMP-3和RASSF1A的表达呈正相关(r=0.256,P<0.05).结论:在大肠癌中TIMP-3和RASSF1A具有明显相关性,TIMP-3和RASSF1A的表达下调可能与大肠癌的发生有关,可以作为鉴别大肠良恶性肿瘤的指标.AIM：To investigate the correlation between TIMP-3 and RASSF1A expression and clinicopathologic features of colorectal cancer.METHODS：The expression of TIMP-3 and RASSF1A was detected by immunohistochemistry in colorectal cancer,tumor-adjacent colorectal tissue and normal colorectal tissue specimens.The correlation between TIMP-3 and RASSF1A expression and clinicopathologic features,such as patient＇s age,gender,infiltration degree,and lymph node metastasis,was analyzed.The correlation between TIMP-3 and RASSF1A expres-sion was also determined by spearman＇s rank correlation analysis.RESULTS：The positive rates of TIMP-3 expression in normal colorectal mucosa,tumor adjacent tissue and colorectal cancer were 90.0%,70.0% and 16.7%,respectively,and those of RASSF1A were 83.3%,63.3% and 23.3%.In colorectal cancer,the expression of TIMP-3 and RASSF1A was related to lymph node metastasis,depth of invasion,and degree of tumor differentiation（all P 0.05）.Spearman rank correlation analysis showed that there is a positive correlation between TIMP-3 and RASSF1A expression in colorectal adenocarcinoma（r = 0.256,P 0.05）.CONCLUSION：The expression of TIMP-3 is signif icantly correlated with that of RASSF1A in colorectal cancer.The down-regulation of TIMP-3 and RASSF1A may be involved in the genesis of colorectal cancer and may be used as an index for evaluation of tumor malignancy.

关 键 词：TIMP-3 RASSF1A 大肠癌 免疫组织化学 

分 类 号：R735.34[医药卫生—肿瘤]