肺内及胸膜腔同期接种人肺腺癌A549细胞建立裸鼠晚期肺癌胸水模型研究  

Study of advanced lung cancer model with effusion in pleural cavity implanted lung adenocarcinoma A549 cells in the lung and pleurai cavity of mice

作　　者：文石兵[1] 夏发明[1] 邓曙光[1] 何林峰[1] 

机构地区：[1]浙江省台州市立医院心胸外科,318000

出　　处：《中华老年医学杂志》2012年第10期906-908,共3页Chinese Journal of Geriatrics

基　　金：浙江省台州市科技局重点项目基金资助（05IKY10）

摘　　要：目的通过裸鼠肺内和胸膜腔移植人肺腺癌A549细胞的方法建立晚期肺癌胸水的动物模型。方法突变系SPF级Balb／c—nu雌性裸鼠分为3组，每组30只，分别于皮下、肺内、肺内及胸膜腔移植肺腺癌A549细胞，观察小鼠的生存时间、体质量、饲料消耗、恶病质状况、肺癌生长和胸水形成的情况。结果裸鼠中位数生存时间肺内及胸膜腔移植、肺内移植均较皮下移植明显缩短，皮下移植生存期范围离散程度明显高于肺内、肺内及胸膜腔移植两组；肺内、肺内及胸膜腔移植两组平均生存时间无差异，平均有效生存时间有差异；肺内、肺内及胸膜腔移植两组小鼠摄食减少、体质量下降和恶病质症状和体征较皮下移植明显，且肺内及胸膜腔移植比肺内移植更多兄。4周内肺内及胸膜腔移植胸水发生率93．3％，而肺内移植仅20．0％出现胸水。结论人肺腺癌A549细胞裸鼠肺内和胸膜腔同期移植模型可作为晚期肺癌胸水的动物模型。Objective To establish the advanced lung cancer model with effusion in the pleural cavity by implanting the lung adenocarcinoma A549 cells into the lung and pleural cavity of mice. Methods The Balb /c nu female mice were divided into three groups and injected with the subculturing lung adenocarcinoma A549 ceils to make three models in the subcutaneous （group A）, the lungs （group B） and the lungs and pleural cavities （group c）. The survival time, weightconsume of feeds, signs and symptoms of cachexia, growth of lung cancer and pleural effusion were observed. Results The median survival time in group C and group B were obviously reduced compared with group A. The range discrete in the group A was higher than group B and group C. There was no significant difference between group B and group C in the average survival time. The effective survival time was significant different between group B and group C. There were differences among three groups in the signs and symptoms of cachexia, the weight and consume of feeds. In 4 weeks, the rate of growing effusion in the pleural cavity was 93.3~ in group C, and only 20.0~ in group B. Conclusions The mice modal implanted the lung adenocarcinoma A549 cells into the lung and pleural cavity of mice in the same time can be as the advanced lung cancer model with effusion in the pleural cavity.

关 键 词：肺肿瘤 胸膜腔 细胞移植 胸水 小鼠 裸 

分 类 号：R734.2[医药卫生—肿瘤]