胃癌组织中miR-155的表达及其临床意义  

MiR-155 expression in gastric cancer tissues and its clinical significance

作　　者：宋军[1] 王辉[1] 付海啸[1] 孟松[1] 徐溢新[1] 徐为[1] 

机构地区：[1]徐州医学院附属医院胃肠外科,江苏徐州221002

出　　处：《中国普通外科杂志》2012年第10期1236-1239,共4页China Journal of General Surgery

基　　金：江苏省卫生厅面上科研项目(H201220);江苏省普通高校研究生科研创新计划项目(CXLX11_0742);徐州市科技发展基金资助项目(XF10C080)

摘　　要：目的:分析胃癌组织中miR-155的表达差异及其临床意义。方法:收集手术切除的52例胃癌标本及其癌旁组织,采用实时荧光定量-聚合酶链反应(RT-PCR)检测癌组织及癌旁组织标本miR-155的表达水平,并分析miR-155的表达与临床病理的关系。结果:RT-PCR结果显示,miR-155在胃癌组织中表达值(0.84±2.27)明显高于其配对癌旁组织中的表达值(0.28±0.56)(P<0.05);miR-155的表达水平与淋巴结转移有关,有淋巴结转移组明显高于无转移组(P<0.05);miR-155的表达与性别、年龄、肿瘤大小、侵犯层次、TNM分期及脉管侵犯无关,各参数的分组间差异无统计学意义(均P>0.05)。结论:miR-155在胃癌组织中呈高表达,且可能与胃癌的淋巴结转移有关。Objective： To investigate the miR-155 expression in gastric cancer tissues and its clinical relevance. Methods： The surgical specimens of 52 cases of stomach cancer and their adjacent normal tissues were collected. The miR-155 expression level in gastric cancer and adjacent normal tissues were determined by real-time reverse transcriptase-polymerase chain reaction （RT-PCR） technique, and the relations of miR-155 expression with the clinicopathologic factors of gastric cancer were analyzed. Results： The RT-PCR results showed that the miR-155 expression value in gastric cancer tissues （0.84＋2.27） was significantly higher than that in their paired ad）acent normal tissues （0.28±0.56） （P〈0.05）. The miR-155 expression level was associated with the lymph node metastases in gastric cancer patients, the miR-155 expression in patients with the lymph node metastases was higher than that in those without lymph node metastases （P〈0.05）. The miR-155 expression level was irrelevant to the sex, age, tumor size, depth of invasion, and all the differences between the groups on these factors had no statistical significance （all P〉0.05）.Conclusion： miR-155 has high expression level in gastric cancer tissues, and it may be relevant to gastric cancer lymph node metastasis.

关 键 词：胃肿瘤 微RNAS MIR-155 淋巴转移 

分 类 号：R735.2[医药卫生—肿瘤]