Dicer在肝细胞癌组织中的表达及其与根治性切除患者预后的关系  

Dicer expression in hepatocellular carcinoma and its association with prognosis in patients subject to radical resection

作　　者：周嘉敏[1] 柴宗涛[1] 王龙蓉[1] 赵一鸣[1] 王苗[1] 孙惠川[1] 樊嘉[1] 王鲁[1] 

机构地区：[1]复旦大学附属中山医院肝肿瘤外科复旦大学肝癌研究所,上海200032

出　　处：《中华实验外科杂志》2012年第10期1889-1891,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30973499）;国家科技重大专项（2008ZX10002-026）

摘　　要：目的探讨Dicer在肝细胞癌和癌周肝组织中的表达及其预后价值。方法检测肝癌患者癌及癌旁冰冻标本及组织芯片中Dicer的表达，分析其表达量与肝癌患者预后的关系。检测Dicer在肝癌和正常肝细胞株中的表达。结果Dicer在肝癌组织中的表达低于癌旁肝组织和正常肝组织（P〈0．01）。癌内低表达患者总生存时间缩短（P〈0．05），癌旁低表达患者总生存时间和无瘤生存时间均缩短（P〈0．05）。Dicer在正常肝细胞株中表达高于肝癌细胞株（P〈0．01），低／无转移潜能肝癌细胞株高于高转移潜能细胞株（P〈0．01）。结论Dicer表达可能与肝癌的发生、肝癌细胞的转移潜能相关。癌组织内及癌旁Dicer低表达提示患者预后差。Objective To evaluate Dicer expression and its prognostic value in patients with hepatocellular carcinoma （HCC） after radical resection. Methods Dicer mRNA expression was detected in HCC frozen specimens （ n = 17） and their adjacent peritumoral tissues. Dicer protein expression was detected in a HCC tissue microarray containing paired intratumoral and peritumoral tissues （ n = 276）, and normal liver tissues （ n = 40）. The analyses were performed to investigate their prognostic values in HCC patients undergoing radical resection. Dicer mRNA and protein expression was further measured in HCC cell lines with different metastatic potentials and a normal liver cell line. Results Dicer mRN. A and protein ex- pression was significantly lower in HCC intratumoral tissues than in their paired peritumoral tissues and normal liver tissues （P 〈 0. 01 ）. HCC patients with lower intratumoral Dicer expression had significantly shorter OS time （ P 〈 0. 05 ） , and patients with lower peritumoral Dicer expression had both significantly shorter OS and DFS time （P 〈 0. 05）. Dicer mRNA and protein expression was higher in L-02 than in HCC cell lines （P 〈 0.01 ）, and it was decreased gradually in HCC cell lines with increased metastatic potentials. Conclusion Dicer expression may be associated with HCC carcinogenesis and metastasis, and its low expression in either intratumoral or peritumoral tissue may indicate poor prognosis in HCC patients after radical resection.

关 键 词：癌 肝细胞 DICER 预后 

分 类 号：R735.7[医药卫生—肿瘤]