RNA干扰对TES基因表达及肿瘤细胞增殖的影响  

RNA interference of the the TES gene expression and tumor cell proliferation

作　　者：王元[1] 董若凡[1] 舒珊[1] 余进进[1] 

机构地区：[1]无锡市第四人民医院妇产科,214062

出　　处：《中华实验外科杂志》2012年第10期2067-2069,共3页Chinese Journal of Experimental Surgery

基　　金：无锡市医管中心医学科技发展基金资助项目（YGM1016）,无锡市科技局科技支撑基金资助项目（CSE01N1113）

摘　　要：目的观察核糖核酸（RNA）干扰对TES基因表达及肿瘤细胞增殖的影响。方法将TES基因特异性的小分子干扰RNA（siRNA）转入肿瘤细胞株Ishikawa，实时荧光定量聚合酶链反应（Real-time PCR）技术检测TESmRNA的表达，Westernblot法进行蛋白定量，噻唑蓝（MTT）比色法检测细胞增殖，研究其对TES基因的表达及细胞增殖的影响。结果RNA干扰后12、24、48、72h，实验组中TES mRNA表达量分别为0．675、0．024、0．324、0．916，较各对照组明显降低，差异均有统计学意义（P〈0．05）；而在不同时间点比较对照A组、对照B组及空白组TESmRNA的表达差异无统计学意义（P〉0．05）。实验组中TESmRNA的表达在24h时最低。RNA干扰后Ishikawa细胞中TES蛋白的表达与TESmRNA水平变化一致，转染后12—48h内，实验组TES蛋白的表达量明显降低分别为65．2、3．2、25．6、33．0，差异均有统计学意义（P〈0．05）；TES蛋白表达在24h时达最低水平6针对TES基因的RNA干扰后Ishikawa细胞的增殖活性明显增强（P〈0．05）。结论针对TES基因的RNA干扰后，TESmRNA和蛋白水平均呈显著下降趋势，同时，Ishikawa细胞的增殖活性明显升高，证实了TES基因在子宫内膜癌中所发挥的抑癌基因的功能。Objective To observe impact of RNA interference of the TES gene expression and tumor cell proliferation. Methods Small interfering RNA （siRNA） specific-targeting TES gene was trans- fected into endometrial cancer cell line Ishikawa, studying its effects on TES expression with＇ quantitative Real-time polymerase chain reaction （Real-time PCR） and western blotting, cellular proliferation was determined by methyl thiazol tetrazolium （MTT） assay. Results At different time point （12, 24, 48, 72 h）, the TES mRNA expression in experimental group were 0. 675, 0. 024, 0. 324, 0. 916, significantly lower compared with control （ P 〈 0. 05 ） ; there was no statistically significant difference between the control groups （P 〉 0.05）. MRNA level of TES reached the lowest at 24 h in the experimental group. TES protein in Ishikawa cells was consistent with TES mRNA levels change after RNA interference： 12-48 h after transfection, the experimental group TES protein expression were 65. 2, 3.2, 25. 6, 33. 0, was signifi- candy reduced, and the differences were statistically significant （ P 〈 0. 05 ） ; TES protein expression was also the lowest at 24 h. After transfection with siRNA, the proliferation activity of Ishikawa ceils was enormously enhanced （P 〈 0.05 ）. Conclusion After RNA interference, TES mRNA and protein levels showed a significant downward trend, proliferation of Ishikawa ceils also was significantly enhanced, confirmed that TES gene functioned as a tumor suppressor in endometrial cancer.

关 键 词：肿瘤 抑癌基因 RNA干扰 

分 类 号：R730.2[医药卫生—肿瘤]