β-环糊精增敏荧光法测定克拉霉素含量的研究  

Determination of Clarithromycin with β-cyclodextrin Sensitized Fluorescence Spectrometry

作　　者：李满秀[1] 张红丽[1] 石轶男[1] 

机构地区：[1]忻州师范学院化学系,山西忻州034000

出　　处：《分析科学学报》2012年第5期701-704,共4页Journal of Analytical Science

基　　金：山西省高校高新技术产业化项目(No.2010020);山西省高等学校教学改革研究项目(2012年)

摘　　要：克拉霉素在λex=275、λem=325处能产生荧光,经β-环糊精增敏后,荧光强度大大增强,据此建立了测定克拉霉素含量的新方法。克拉霉素的浓度在5.0×10-7～5.0×10-6 mol/L范围内与体系荧光强度呈良好的线性关系,相关系数R=0.9992,检出限为3.9×10-7 mol/L。对药物制剂中克拉霉素的含量进行测定,测定值与标示值吻合,回收率在97%以上。该方法可用于测定胶囊和血清样品中克拉霉素的含量。Clarithromycin fluorescence was at λex = 275 nm,λem = 325 nm, and its fluorescence intensity could be enhanced greatly by p-cyclodextrins. Based on this fact, a new method for measuring clarithromycin is established. A linear relationship between the concentration of clarithromycin and fluorescence intensity was obtained in the range of 5.0× 10^-7-5.0 × 10^-6 mol/L（R = 0. 9992） with the detection limit of 3.9× 10^-7 mol/L. For the determination of erythromycin in pharmaceutical preparations, the measured result was coincided with the indicated value.

关 键 词：增敏荧光法 克拉霉素 Β-环糊精 包结常数 

分 类 号：O657.39[理学—分析化学]