新生血管特异性结合肽GX1抑制小鼠视网膜新生血管生成的实验研究  

A novel peptide(GX1) homing to neovasculature inhibits retinal neovascularization in mice

作　　者：方如塘[1] 惠晓丽[2] 殷继鹏[1] 吴开春[1] 

机构地区：[1]第四军医大学西京消化病医院肿瘤生物学国家重点实验室,西安710032 [2]西安交通大学医学院第一附属医院

出　　处：《陕西医学杂志》2012年第10期1275-1277,1301,共4页Shaanxi Medical Journal

基　　金：国家自然科学基金重大项目(81090270;81090273);国家自然科学基金(30900704);国家科技重大专项"重大新药创制"2009ZX09103专题;国家高技术研究发展计划(863计划)(No.2006AA022103)

摘　　要：目的:观察GX1短肽对小鼠视网膜新生血管有无特异性结合及对其生成的作用。方法:采用高氧诱导小鼠视网膜新生血管生成,免疫组化检测小鼠视网膜新生血管有无GX1短肽受体表达,并比较GX1作用组与对照组视网膜新生血管内皮突破视网膜内界膜的细胞核数目及视网膜铺片血管密度的差异。结果:GX1短肽能与小鼠视网膜新生血管特异性结合,且GX1作用后,高氧诱导小鼠视网膜新生血管密度及迂曲程度较对照组减低,突破视网膜内界膜的内皮细胞核数目减少。结论:GX1短肽具有抑制小鼠视网膜新生血管生成的作用。Objective：To verificate the GX1 binding specificity to retinal neovasculature and investigate the effects of GX1 on retinal neovaseularization in mice. Methods：After oxygen-induced retinopathy mice model was es- tablished, the model mice were then intraperitoneally injected GX1 or nothing for five days. The effects of GX1 on retinal neovascularization were evaluated by counting the endothelial nuclei of new vessels extending from the retina to the vitreous in histological sections with H^E staining. Retinal flat mount was used to observe the changes of ret- inal vessels. Immunohistochemistry was adopted to verificate the GX1 binding specificity to retinal neovascuiature. Results：By immunohistochemistry GX1 was shown to bind specifically to retinal neovasculature. The number of the endothelial nuclei of new vessels extending from the retina to the vitreous was significantly less in the model mice with GX1 injection than that in the model mice without GX1 injection. In the retinal flat mounts,the density and ab- normality of retinal new vessels was noted much less in the model mice with GX1 injection compared to that of the model mice without GX1. No obvious changes were found in normal control group. Conclusion： Besides the GX1 binding specificity to retinal neovasculature,GX1 have the effects to inhibit retinal neovascularization in mice.

关 键 词：视网膜血管 血管生成抑制剂 血管抑制蛋白质类 @GX1 

分 类 号：R774.1[医药卫生—眼科]