3种天然产物中黄酮类化合物总含量的测定  

Determination of total flavonoids in three natural products

作　　者：韩秀萍[1] 田海燕 付云芝[1] 黄仙红[1] 王丽芝[1] 

机构地区：[1]海南大学材料与化工学院,海口570228 [2]山西省孝义市孝义三中,孝义032300

出　　处：《食品科技》2012年第10期264-266,272,共4页Food Science and Technology

基　　金：国家自然基金(90207026);海南省自然基金(211009);海南大学与天津大学合作项目

摘　　要：采用紫外-可见分光光度法测定3种天然产物:黄秋葵叶、水稻叶及刺苋中总黄酮类化合物的含量。用70%乙醇超声提取3种天然产物中的黄酮类化合物,以芦丁为对照品,以NaNO2-Al(NO3)3-NaOH为显色系统,在505nm处进行分光光度测定,建立标准曲线A=0.01175C-0.02247,相关度r=0.9998。结果表明,3种天然物中黄酮类化合物总含量分别为黄秋葵叶:34.40mg/g;刺苋:20.26mg/g;水稻叶:2.34mg/g。The flavonoids in three natural products （including okra leaves, rice leaves, Theorny amaranth） were determined by UV-visible spectrophotometry. The flavonoids in three natural products were ultrasonic extracted with 70% ethanol. The standard curve A=0.01175C-0.02247 of Rutin was extablished by spectrophotometry at 505 nm when the color reagent was admixtures of NaNO2-Al（NO3）3-NaOH solution. Data between the flavonoids content and absorbance had a better linear relationship r=0.9998. The total flavonoids in three natural products are okra leaves 34.40 mg/g, rice leaves 20.26 mg/g, Theorny amaranth 2.34 mg/g.

关 键 词：黄秋葵叶 水稻叶 刺苋 黄酮类化合物 分光光度法 

分 类 号：TS207.3[轻工技术与工程—食品科学]