1株1,2-二氯乙烷降解菌的分离及降解特性研究  

Isolation,Degradation Characteristics of a 1,2-Dichloroethane-Degrading Strain

作　　者：王小春[1] 陈东之[1] 金小君[1] 陈建孟[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,杭州310032

出　　处：《环境科学》2012年第10期3620-3626,共7页Environmental Science

基　　金：国家自然科学基金项目(51178430);浙江省创新团队项目(20110150)

摘　　要：从浙江某化工厂污水处理池的活性污泥中筛选分离到1株能以1,2-二氯乙烷(1,2-dichloroethane,1,2-DCA)为唯一碳源和能源生长的菌株T-2,根据菌株的形态特征、生理生化特性及16S rRNA基因序列分析,该菌株被鉴定为Starkeya sp.研究表明,Starkeya sp.T-2生长和降解1,2-DCA较适宜的温度为30℃,较适宜的pH为7.0～8.0;菌株降解1,2-DCA的过程遵循Haldane动力学模型,其最大比生长速率μmax为0.065 h-1,最大比降解速率vmax为0.54 h-1;菌株降解1,2-DCA的耐受浓度为500 mg.L-1,细胞产率系数(以1,2-DCA计)为0.191 mg.mg-1,菌株T-2利用1,2-DCA生长的过程中能同时把1,2-DCA最终矿化为CO2和H2O,矿化率为45%.A 1,2-dichloroethane-degrading bacterium T-2,which was able to utilize 1,2-dichloroethane as the sole carbon and energy source,was isolated from the activated sludge of a chemical plant in Zhejiang.According to the morphological,physiological and biochemical characteristics and sequence analysis of 16S rRNA,the strain was identified as Starkeya sp.T-2.The optimal temperature and pH for 1,2-DCA degradation were 30℃ and 7.0-8.0,respectively.The 1,2-DCA degrading process followed the Haldane kinetic model.The maximum specific growth rate and specific degradation rate were 0.065 h-1 and 0.54 h-1,respectively.The maximum tolerable concentration of 1,2-DCA for the strain was 500 mg·L-1,and the cell yield coefficient was 0.191 mg·mg-1.Strain T-2 could mineralize 1,2-DCA into CO2 and H2O,with a mineralization ratio of 45%.

关 键 词：1 2-二氯乙烷 分离鉴定 降解特性 动力学 碳平衡 

分 类 号：X172[环境科学与工程—环境科学]