活血定痛酊的质量标准研究  

Quality Standard of Huoxue Dingtong Tincture

作　　者：杨璐璐[1] 秦兴卫 宁亚功[1] 彭静[1] 邓富[1] 

机构地区：[1]成都军区昆明总医院,昆明650032 [2]昆明赛诺制药有限公司,昆明650021

出　　处：《中国药房》2012年第39期3703-3706,共4页China Pharmacy

基　　金：全军医药卫生科研基金课题(2006071001)

摘　　要：目的:建立活血定痛酊的质量标准。方法:采用薄层色谱(TLC)法对方中川芎、骨碎补、马钱子进行定性鉴别;采用高效液相色谱法对制剂中乌头碱和阿魏酸进行含量测定。结果:TLC斑点清晰、分离度好、专属性强;乌头碱的进样量在0.18～1.08μg(r=0.9999)范围内与峰面积积分值呈良好线性关系,平均回收率为97.17%,RSD=1.31%(n=9);阿魏酸检测浓度在5.2～15.7μg.mL-1(r=0.9998)范围内与峰面积积分值呈良好线性关系,平均回收率为97.51%,RSD=1.40%(n=9)。结论:所建标准可用于活血定痛酊的质量控制。OBJECTIVE：To establish the quality standard of Huoxue dingtong tincture.METHODS：TLC method was used for the identification of Ligusticum chuanxiong,Drynaria fortunei and Strychnos nuxvomica;HPLC method was adopted to determine the contents of aconitine and ferulic acid.RESULTS：TLC spots were clear,well-separated and specific.The linear range of aconitine was 0.18～1.08 μg（r=0.999 9）with an average recovery of 97.17%（RSD=1.31%,n=9）.The linear range of ferulic acid was 5.2～15.7 μg.mL-1（r=0.999 8）with an average recovery of 97.51%（RSD=1.40%,n=9）.CONCLUSION：The method can be used for the quality control of Huoxue dingtong tincture.

关 键 词：活血定痛酊 川芎 骨碎补 马钱子 乌头碱 阿魏酸 

分 类 号：R284.2[医药卫生—中药学] R283.662[医药卫生—中医学]