HPLC法测定青城山野生蕨菜中总黄酮的含量  

Content Determination of Total Flavones in Pteridium aquilinum from Qingchengshan by HPLC

作　　者：李航[1] 李鹏[1] 张勇[1] 

机构地区：[1]解放军第452医院,成都610021

出　　处：《中国药房》2012年第39期3712-3714,共3页China Pharmacy

摘　　要：目的:建立测定青城山野生蕨菜中总黄酮含量的方法。方法:蕨菜干粉经提取水解后通过高效液相色谱法测定其中槲皮素和山柰酚的含量,从而确定总黄酮的含量。色谱柱为ZorbaxSB-C18(150mm×4.6mm,5μm),流动相为甲醇-0.4%磷酸溶液(52:48,V/V),流速为1.0mL.min-1,检测波长为254nm,柱温为(30±1)℃。结果:槲皮素进样量在0.204～2.040μg范围内与峰面积积分值呈良好线性关系(r=0.9996),平均加样回收率为98.28%,RSD=4.27%(n=6);山柰酚进样量在0.244～2.440μg范围内与峰面积积分值呈良好线性关系(r=0.9999),平均加样回收率为99.46%,RSD=4.82%(n=6)。结论:本方法准确、可靠、重复性好,可作为蕨菜总黄酮含量测定方法,为蕨菜的药用开发利用提供依据。OBJECTIVE：To establish the method for the content determination of total flavones in Pteridium aquilinum form Qingchengshan.METHODS：The contents of quercetrin and kaempferol were determined by HPLC after hydrolysis.The determination was performed on Zorbax SB-C 18（150 mm×4.6 mm,5 μm）column with mobile phase consisted of methanol-0.4%phosphoric acid（52：48,V/V）at the flow rate of 1.0 mL.min-1.The detection wavelength was set at 254 nm and column temperature was（30±1）℃.RESULTS：The linear range of quercetrin was 0.204～2.040 μg（r=0.999 6）with an average recovery of 98.28%（RSD=4.27%,n=6）;that of kaempferol was 0.244～2.440 μg（r=0.999 9,n=6）with an average recovery of 99.46%（RSD= 4.82%,n=6）,respectively.CONCLUSION：The method is accurate,reliable and reproducible.It can be used for the content determination of total flavonoids in P.aquilinum and provides reference for the development of P.aquilinum.

关 键 词：野生蕨菜 总黄酮 槲皮素 山柰酚 含量测定 高效液相色谱法 青城山 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]