玉米秸秆高效分解菌株的筛选、鉴定及产酶条件优化  

Screening,identification and cultural condition optimization of corn straw efficient decomposition strains

作　　者：杨琼[1] 孙满吉[1] 黄立敏[1] 王寅[1] 朱继红[1] 徐春桃[1] 李建平[1] 

机构地区：[1]东北农业大学

出　　处：《中国饲料》2012年第19期31-33,37,共4页China Feed

基　　金：奶牛高产新型调控剂开发与利用(12521032)

摘　　要：为了从瘤胃液中筛选出一株对玉米秸秆分解能力较强的纤维菌,试验采用稀释平板法分离奶牛瘤胃菌,通过刚果红平板法对产纤维素酶的菌株进行初筛,选取透明圈大的菌株进行玉米秸秆摇瓶复筛,得到一株对玉米秸秆分解率最高的菌株。根据菌株形态、生理生化特征对菌株进行初步鉴定,并对该菌株的产酶条件进行了优化。结果表明,该菌株为芽孢杆菌属,接种量为8%、氮源为NH3NO3、吐温80含量1%为该菌最适产酶条件。In order to screen out a cellulose strain with a higher decomposition rate on corn straw from rumen fluid,the bacteria from the rumen fluid of cows were isolated by the pour plate method,the strain which could produce cellulase preliminarily was screened by Congo red medium plate method and flask shaking fermentation method.A strain with a higher decomposition rate on corn straw was the purpose strain.According to morphological and physiological characters,the strain was identified as Bacillus sp.The results showed that：the optimum conditions for enzyme production were inoculation quantity 8 %,NH4NO3 as nitrogen source,tween-80 concentration 1 %.

关 键 词：玉米秸秆 纤维素酶 筛选 鉴定 产酶条件 

分 类 号：S816.17[农业科学—饲料科学]