大鼠脑缺血再灌注损伤后血清MIP-3α与IL-6、TNF-α的关系  

The relationship between the MIP-3α and IL-6 and TNF-α after cerebral ischemia-reperfusion injury in rats

作　　者：王春雷 朵慧敏[2] 杨春晓[3] 

机构地区：[1]哈尔滨市第二医院,黑龙江哈尔滨150056 [2]哈尔滨医科大学附属第二医院物理诊断室,黑龙江哈尔滨150001 [3]哈尔滨医科大学附属第二医院神经内科,黑龙江哈尔滨150001

出　　处：《中风与神经疾病杂志》2012年第9期824-827,共4页Journal of Apoplexy and Nervous Diseases

摘　　要：目的研究巨噬细胞炎性蛋白-3alpha(MIP-3α)、IL-6和TNF-α在大鼠局灶性脑缺血再灌注损伤中给予中和抗体前后不同时间点的表达;探讨CC亚族趋化因子MIP-3α与IL-6和TNF-α的关系。方法 Wistar大鼠66只随机分为3组:假手术对照组6只、缺血-再灌注损伤组30只;MIP-3α中和抗体组30只。缺血再灌注损伤组和MIP-3α中和抗体组均按缺血再灌注后不同时间点再分为5组,每组6只。采用线拴法制作大鼠大脑中动脉缺血模型(MCAO),于缺血90min后再灌注,中和抗体组于再灌注同时腹腔注射MIP-3α中和抗体5μg/100g大鼠,分别于不同时间点采取大鼠静脉血,ELISA法检测血清中MIP-3α、IL-6和TNF-α。结果缺血再灌注损伤组MIP-3α、IL-6、TNF-α于再灌注后各时间点的表达明显高于对照组;MIP-3α中和抗体组的MIP-3α于各时间点表达较缺血再灌注组明显降低;IL-6、TNF-α于缺血再灌注后3h、6h依然升高,与缺血再灌注组相同时间点比较(P>0.05),差别无统计学意义;而12h两者增高则显著减慢,以后至48h一直下降。结论 IL-6和TNF-α可能是诱导了MIP-3α的产生;而MIP-3α又趋化IL-6和TNF-α炎性因子的表达从而形成恶性循环加重脑缺血再灌注损伤。Objective To study the macrophage inflammatory-3alpha（MIP-3α）,IL-6 and TNF-α expression of rat focal cerebral ischemia-reperfusion injury in serum,and to explore the relationship between CC subfamily of chemokines MIP-3α and IL-6 and TNF-α.Methods 66 Wistar rats were randomly divided into three groups.Sham-operated control group had 6 rats,Ischemia-reperfusion injury group had 30 rats,and MIP-3α neutralizing antibodies group had 30 rats.The Ischemia-reperfusion injury group and MIP-3α neutralizing antibodies group was subdivided after ischemia-reperfusion into five groups.The focal cerebral ischemia-reperfusion by MCAO was induced according to Longa＇s suture method.The reperfusion formed after 90 minutes of ischemia.The ischemia-reperfusion injury group at the reperfusion time also injected MIP-3α neutralizing antibody 5μg/100g.Respectively at different time points,rats＇ blood was taken.Enzyme linked immunosorbent assay method was used for the detection of MIP-3α expression.Same method was used to detect the IL-6 and TNF-α.Results The expression of MIP-3α,IL-6 and TNF-α after ischemia-reperfusion was significantly higher than that of sham-operated control group.The expression of MIP-3α after ischemia-reperfusion in MIP-3α neutralizing antibodies group was significantly decreased.The IL-6 and TNF-α in ischemia-reperfusion remained elevated in 3h and 6h.But it significantly slowed down within 12 hours,and continued up to 48 hours.Conclusion IL-6 and TNF-α may induce the production of MIP-3α;and the MIP-3α has chemokine IL-6 and TNF-α expression of inflammatory factors and thus form a vicious cycle of increasing cerebral ischemia-reperfusion injury.

关 键 词：巨噬细胞炎性蛋白-3α MIP-3α中和抗体 白介素-6 肿瘤坏死因子-α 脑缺血再灌注 

分 类 号：R743.3[医药卫生—神经病学与精神病学]