具斑芒毛苣苔抑制α-葡萄糖苷酶活性成分研究  

α-Glucosidase inhibitory activity of Aeschynanthus maculatus

作　　者：田璞玉[1] 康文艺[1] 

机构地区：[1]河南大学中药研究所,河南开封475004

出　　处：《中国中药杂志》2012年第19期2910-2912,共3页China Journal of Chinese Materia Medica

摘　　要：目的:研究具斑芒毛苣苔抑制α-葡萄糖苷酶的活性成分。方法:用体外α-葡萄糖苷酶抑制模型筛选;采用各种色谱法对高活性部位分离,运用多种波谱技术鉴定结构。结果:从具斑芒毛苣苔分离得到7个化合物,分别为羽扇豆醇(1),豆甾醇(2),熊果酸(3),豆甾-5,22(E)-二烯-3β-醇(4)和β-胡萝卜苷(5),3-hydroxy-12-taraxasten-28-oic-acid(6),齐墩果酸(7)。化合物1(IC50 25.41 mg.L-1),3(IC50 4.42 mg.L-1),4(IC50 11.50 mg.L-1),6(IC50 14.17 mg.L-1)和7(IC50 2.88mg.L-1)的体外抑制α-葡萄糖苷酶活性高于阳性对照药物acarbose(IC501 103.01 mg.L-1)。结论:化合物1～7为首次从该植物中分离得到,6为首次从该科中分离得到,7为首次从该属植物中分离得到。Objective： To study the inhibitory activity of Aeschynanthus maculatus on α-glucosidase. Method： The inhibilitory model of in vitro α-glucosidase was established. Active extracts of A. maculatus were isolated and identified bymultiple chromatographic methods, and their molecular structures were identifiied by spectral techniques. Result： Seven coumpounts were isolated from A. maculatus and isolated as lupeol（1）, stigmasterol（2）, ursolic acid（3）, stigmast-5,22（E）-diene-3β-ol（4）, β-daucosterol（5）, 3-hydroxy-12-taraxasten-28-oic-acid（6） and oleanic acid（7）. Compounds 1 （IC50 25.41 mg·L-1）, 3 （IC50 4.42 mg·L-1）, 4 （IC50 11.50 mg·L-1）, 6 （IC50 14.17 mg·L-1） and 7 （IC50 2.88 mg·L-1）had higher inhibitory activities than that of acarbose（IC501 103.01 mg·L-1） as the positive control drug. Conclusion： Compound 1-7 were isolated from this plant for the first time. Compound 6 was isolated from Gesneriaceae family for the first time. Compound 7 was isolated fromAeschynanthus genus for the first time.

关 键 词：具斑芒毛苣苔 Α-葡萄糖苷酶 

分 类 号：R284[医药卫生—中药学]