丝状蓝藻Calothrix sp.PCC7601原生质球的分离和再生  

Spheroplast Isolation and Regeneration in the Filamentous Cyanobacterium Calothrix sp.PCC7601

作　　者：章军[1] 徐虹[1] 蔡晖 楼士林[1] 

机构地区：[1]厦门大学生物学系细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2000年第3期381-385,共5页Journal of Xiamen University：Natural Science

基　　金：:国家自然科学基金!(39570 4 0 7);86 3计划资助项目!(819- 0 4 - 0 3)

摘　　要：采用溶菌酶、纤维素酶和果胶酶混合处理法 ,首次较好地分离具活力的丝状蓝藻 Calothrixsp.PCC76 0 1的原生质球 .通过再生培养 ,跟踪显微观察了原生质球的再生过程 ,并且获得了再生藻株 .为丝状蓝藻的转基因工作提供了一种潜在的受体细胞 .Viable spheroplasts were first isolated enzymatically form the filamentous cyanobacterium Calothrix sp.PCC76 0 1 ,by using a mixture of lysozyme,cellulase and pectolyase.Developmentof spheroplast in culture of was observed periodically by microscope and whole filaments were obtained.This study offers a potential hostcell for gene transfer of foreign DNA into filamentous cyanobacteria.

关 键 词：丝状蓝藻 原生质球 分离 再生 转基因工程 

分 类 号：Q949.221[生物学—植物学]