响应面分析法优化回流提取岷江柏总黄酮工艺  

Optimization of Reflux Extraction Process for Total Flavonoids from Cupressus chengjana S.Y.Hu by Response Surface Methodology

作　　者：王文兰[1] 杨梅[1] 严平[1] 王晓玲[1] 

机构地区：[1]西南民族大学少数民族药物研究所,四川成都610041

出　　处：《食品科学》2012年第18期57-62,共6页Food Science

基　　金：西南民族大学中央高校基本科研业务费项目(11NFW02);西南民族大学2012年研究生学位点建设项目(2012XWD-S0703)

摘　　要：目的:优化岷江柏总黄酮的回流提取工艺。方法:在单因素试验的基础上,采用响应面分析法,以总黄酮提取率为响应值,通过回归分析各工艺参数与响应值之间的关系,优化岷江柏总黄酮最佳提取工艺条件。结果:最佳工艺条件为回流温度90℃、乙醇体积分数70%、浸泡时间37min,其他条件为回流时间70min、液料比40:1(mL/g)、提取次数3次,在最佳工艺条件下提取率可达7.211%。结论:响应面分析法优化回流提取总黄酮的预测准确、方便,所得的最佳提取工艺高效、可行。Objective： To optimize the reflux extraction of total flavonoids from leaves and stems of Cupressus chengjana S. Y. Hu. Methods： One-factor-at-a-time design and response surface methodology （RSM） were used to determine optimal conditions for the extraction of total flavonoids through regression analysis of the relationship between the extraction efficiency of total flavonoids and various process parameters. Results： The optimal extraction process was soaking for 37 min in 70% ethanol solution at a solid-to-solvent ration of 1：40 （g/mL） followed by reflux extraction repeated 3 times for 70 min each time, resulting in an extraction efficiency of 7.211%. Conclusion： E esponse surface methodology can provide an accurate and convenient way to predict the reflux extraction of total flavonoids to obtain optimal process with high effectiveness and applicability.

关 键 词：响应面法 岷江柏 总黄酮 回流提取 

分 类 号：TQ461[化学工程—制药化工]