一种新型咔唑类菁染料黏度荧光探针  

A Novel Carbazole-based Cyanine as a Fluorescent Probe for Viscosity Detection

作　　者：刘飞[1] 吴彤[1] 胡明明[1] 彭孝军[1] 樊江莉[1] 

机构地区：[1]大连理工大学精细化工国家重点实验室,大连116024

出　　处：《高等学校化学学报》2012年第10期2239-2243,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:21136002;21076032;20923006)资助

摘　　要：合成了一种带有醛基的咔唑类菁染料KQ,其自身的荧光量子产率较低,对极性的敏感性小,且荧光信号不受核酸等生物分子的干扰.KQ对环境黏度有很好的荧光响应,相对荧光强度随着环境黏度的增大而增强,并且在1×10-3～1.3216 Pa·s黏度范围内,染料的荧光强度与溶液的黏度呈良好的线性关系.活细胞荧光成像实验结果表明,染料KQ具有良好的细胞膜通透性,并可对细胞内不同位置的黏度检测成像.KQ, a carbazole-based cyanine dye with aldehyde, was synthesized. Dye KQ showed lower fluo- rescence quantum yield and no obvious fluorescence responses to the polarity of solvents and bio-macromole- cules such as nucleic acids. Dye KQ was very sensitive to the environmental viscosity and gave strong fluores- cence enhancement with the increase of the viscosity. Notably, there is a linear relationship between them. The live cell imaging experiments manifested that KQ could penetrate the cell membrane easily, detect the vis- cosity of the cellular micro-environment and gave bright and explicit staining image of the inner membrane sys- tem of live cells.

关 键 词：咔唑类菁染料 黏度 荧光探针 活细胞成像 

分 类 号：O652[理学—分析化学]