复合诱变抗性筛选法选育高转化甘油产1,3-二羟基丙酮菌株  

Screening of Mutant Strains for Converting Glycerol to Dihydroxyacetone by UV and ^(60)Co Irradiation Combining Drug-Resistance Technique

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作  者:戴甄[1] 张波[1] 王刚[2] 蓝小玲[1] 杨晓琴[2] 魏屹[1] 李学如[1] 

机构地区:[1]西南交通大学生命科学与工程学院,四川成都610031 [2]四川省食品发酵工业研究设计院,四川成都611130

出  处:《化学与生物工程》2012年第10期25-27,57,共4页Chemistry & Bioengineering

基  金:四川省自然科学基金资助项目(2010SZ0213);中央高校基本科研业务费专项资金资助项目(SWJTU11ZT25)

摘  要:在测定不动杆菌(Acinetobacter sp.)Asp16菌株链霉素最低杀菌浓度的基础上,对菌株进行UV和60 Co诱变处理后,利用链霉素抗性平板筛选获得1株遗传性能稳定、高转化甘油产1,3-二羟基丙酮的突变株Asr168,将突变株在5.0g·L-1酵母膏、140.0g·L-1甘油、7.0g·L-1 CaCO3组成的发酵培养基中于30℃、250r·min-1摇床培养50h,1,3-二羟基丙酮的产量达到136g·L-1,比出发菌株提高200%。表明通过UV和60 Co诱变,结合链霉素抗性平板筛选,可大幅提高不动杆菌转化甘油产1,3-二羟基丙酮的能力。Ni-Ce-B Amorphous alloy was prepared at 50 *C by the reduction of NiCl2 6H20 and Ce(NO3)3 6HzO using KBH4 as reduction agent in OP-lO-SDS/n-butanol/cyclohexane/water microemulsion, and its morphology,structure, thermal stability and crystallization properties were studied by TEM, SEM, TG-DSC and XRD. The effect of size of aqueous nucleus on average particle size was investigated through changing the composition of aqueous phase. The results showed that Ni-Ce-B amorphous alloy with average particle size of 30 nm could be prepared,and the particle size distribution was uniform with good monodispersity. The average particle size increased with w value and Rw value increasing. The crystallization temperature of Ni-Ce-B amor- phous alloy was significantly higher than that of Ni-B amorphous alloy by 360 ℃.

关 键 词:1 3-二羟基丙酮 不动杆菌 高产突变株 抗性筛选 

分 类 号:TQ224.6[化学工程—有机化工] Q939[生物学—微生物学]

 

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