超声定位辐照载血卟啉微泡治疗兔肝VX2肿瘤的实验研究  

Effect of ultrasound combined with microbubbles loaded hematoporphyrin for treatment of rabbit liver VX2 tumor

作　　者：张勇[1] 王志刚[2,1] 康娟[3] 冉海涛[1] 任建丽[1] 李攀[2] 

机构地区：[1]重庆医科大学附属第一医院超声科,重庆400016 [2]重庆医科大学超声影像学研究所,重庆400010 [3]重庆医科大学附属第二医院肝病研究中心,重庆400010

出　　处：《中国介入影像与治疗学》2012年第10期757-761,共5页Chinese Journal of Interventional Imaging and Therapy

基　　金：国家自然科学基金(81071158)

摘　　要：目的探讨超声定位辐照载血卟啉微泡介导药物靶向释放技术治疗兔VX2肝移植瘤的效果。方法利用35只新西兰大白兔建立肝VX2移植瘤模型,并随机均分为超声定位辐照载血卟啉微泡组(US+LMLH组)、超声定位辐照血卟啉组(US+HP组)、超声定位辐照空白脂质微泡组(US+MB组)、单纯载血卟啉微泡组(LMLH组)、单纯血卟啉组(HP组)、单纯超声辐照组(US组)和生理盐水对照组(C组)。治疗前后用二维超声、CDFI及CEUS观察肿瘤大小、回声及血流灌注情况,计算肿瘤体积大小及生长抑制率;同时观察不同处理组肝内转移及远处转移情况;并用透射电镜观察肿瘤细胞的超微结构。结果US+LMLH组肿瘤内部呈混合回声,CDFI及超声造影显示肿瘤的滋养血管明显减少,生长抑制率高于其他各组;在肝内及远处转移方面,US+LMLH组较少转移,明显优于其他各组;超微结构显示US+LMLH组细胞膜破坏,线粒体明显肿胀。结论超声定位辐照载血卟啉微泡能有效激活血卟啉,具有较强的体内抑瘤效果。Objective To explore the anti-tumor effects of lipid microbubbles loaded hematoporphyrin （LMLH） on rabbit liver VX2 tumor using ultrasound mediated target drug delivery. Methods Thirty-five New-Zealand rabbits were induced into liver VX2 tumor and randomly divided into 7 groups （each n=5）, i.e. Ultrasoundq-LMLH group （US＋LMLH）, Hematoporphyrin （HP） ＋ultrasound group （US＋HP）, ultrasound ＋ lipid mierobubbles group （US＋MB）, LMLH a- lone group （LMLH）, HP alone group （HP）, US alone group （US） and normal saline （NS） alone as control group （C）. Ultrasonography, including 2D, CDFI and CEUS were applied to detect the size, echo and blood of tumor, and the tumor growth inhibiting rate was calculated. The tumors were harvested to observe in transmission electron microscope, while the intrahepatic metastasis and distant metastasis were observed. Results The tumors in US＋LMLH group had mixing echo. CDFI and CEUS showed that the blood vessels obviously decreased, and the tumor growth inhibiting rate was higher in US ＋LMLH than in other groups. There were less metastases in US＋LMLH group than in other groups. Ultramicrostruc- ture showed cellular membrane was broken, and mitochondria were obviously swell. Conclusion HP can be efficiently acti- vated by ultrasound. This technique can inhibit tumor growth significantly in vivo.

关 键 词：超声检查 微泡 血卟啉 声动力疗法 肝肿瘤 

分 类 号：R735.7[医药卫生—肿瘤] R445.1[医药卫生—临床医学]