胃结肠癌患者血清TK1的分布及诊断价值  

The distribution and diagnostic value evaluation of serum thymidine kinase 1 in gastricand colonic cancer patients

作　　者：汪圣毅[1] 陈志武[2] 张丹[2] 郑立平[1] 宋景戈[1] 姜皖杰[1] 刘弋[1] 

机构地区：[1]安徽医科大学第一附属医院普外科,合肥230022 [2]安徽医科大学基础医学院药理学教研室,合肥230032

出　　处：《国际医药卫生导报》2012年第20期2939-2942,共4页International Medicine and Health Guidance News

基　　金：2011年度安徽省博士后研究人员科研活动经费资助项目;安徽高校省级自然科学研究项目（KJ20122180）

摘　　要：目的探讨胃结肠癌患者血清胸苷激酶1（Thymidine kinase 1，TK1）的分布情况和诊断价值。方法免疫印迹增强化学发光法检测70例胃结肠癌患者（研究组）、48例健康体检者（对照组）的血清TK1值，分析分布形态，计算基于参考值的阳性率，比较集中趋势，ROC曲线分析诊断价值。结果研究组与对照组的偏度系数分别为4．975、0．836，研究组的阳性率高于对照组（37．14％VS4．17％，P〈0．01），研究组的中位数大于对照组（1．56VS0．91，P〈0．01），ROC曲线下面积为0．7987，临界值为2．025pmol／L时，阳性似然比为最大值17．83，灵敏度、特异度分别为37．14％、97．92％。结论胃结肠癌患者的TK1值呈正偏分布，可能预示癌细胞和宿主细胞的增殖状态差异，其阳性率和中位数均高于正常对照组，参考值为2．025pmol／L的诊断价值较高。Objective To explore the distribution charateristics and evaluate the diagnostic ralue of serum thymidine kinase 1 （TK1） in the gastric and colonic cancer patients. Methods 70 cases of ;astric and colonic cancer patients（observation group） ,48 cases of healthy people（control group） under- ~ent serum TK1 detection using an ECL dot blot assay, TKI＇s statistical distribution, positive rate and ＇,entral tendency were analyzed and compared between two groups, its clinical diagnostic value was evalu- ated using receiver operating characteristic （ROC） curve. Results The coefficients of skewness were 4. t75,0.836 in observation group and control group respectively, the positve rate in observation group was ignificantly higher than that in control group（37.14% vs 4.17%, P〈 0.01）, the TK1 median in observa- ion group was also significantly higher than that in control group（1.56 vs 0.91, P〈 0.01）, ROC analysis howed that the area under curve （AUC） was 0.7987. When the cutoff value was 2.025 pmol/L, the positive ikelihood ratio was the biggest（17.83）, then the sensitivity and specificity were 37.14% ,97.92% respectively. Conclusions TK1 values in gastric and colonic cancer patients are not normal distribution, its outliers aay imply the proliferation difference of both cancer and non-cancer cells, its positive rate and median are ignificantly higher than those of healthy people, its diagnostic value is best when cutoff value is 2.025 ,mol/L.

关 键 词：胸苷激酶1(TK10) 胃癌 结肠癌 诊断 

分 类 号：R735[医药卫生—肿瘤]