废弃活性污泥中产聚羟基脂肪酸酯菌株Bacillus sp. PB-3的发酵条件优化  

Optimization of PHAs production by Bacillus sp. PB-3 from waste activated sludge

作　　者：郝大可[1,2] 李强[2] 韩省[3] 王佳佳[3] 罗琰[2] 宫恺[2] 

机构地区：[1]山东省医学科学院,济南250062 [2]济南大学山东省医学科学院医学与生命科学学院,济南250022 [3]济南大学化学化工学院,济南250022

出　　处：《生物加工过程》2012年第5期28-33,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金资助项目(31100088);公安部应用创新计划资助项目(2010YYCXSDST054)

摘　　要：通过尼罗红染色法结合荧光显微镜镜检,从废弃活性污泥中分离得到1株高产聚羟基脂肪酸酯(PHAs)的菌株Bacillus sp.PB-3,经气相色谱法鉴定该菌株胞内产物为聚β-羟基丁酸酯(PHB)。对培养基成分及发酵条件优化后,获得最佳培养方案:12 g/L的葡萄糖为C源,2 g/L的牛肉膏为N源,初始pH 7.5,培养基装液量80 mL,转速为200 r/min,37℃培养48 h,PHB质量分数可达菌体干质量的32.09%,比优化前提高30%。A high-yield strain of PHAs, Bacillus sp. PB-3 was isolated from waste activated sludge by Nile red staining and fluorescence microscopy screening. Gas chromatography analysis indicated that the intracellular production of the strain was polyhydroxybutyrate （ PHB）. Furthermore, medium composition and fermentation factors were investigated and optimized. The optimal carbon and nitrogen sources were glucose （12 g/L） and beef paste （2 g/L）, respectively. The optimal cultivation conditions were pH 7.5, 37 ℃ for 48 h, and 80 mL medium at an agitation rate of 200 r/min. Under the fermentation conditions, the yield of PHB was raised to 32.09% （w/w） 30% higher than that before optimized

关 键 词：聚羟基脂肪酸酯 聚羟基丁酸酯 筛选 发酵 

分 类 号：Q936[生物学—微生物学]