高饱合脂肪酸饮食下调大鼠肝脏和脂肪胰岛素信号通路的基因表达  

Down-regulated expression of insulin signal pathway in liver and fat of rats induced by high saturated fatty acid diet

作　　者：崔秋英[1] 贾敏[1] 霍丽静[1] 赵晓云[1] 

机构地区：[1]河北省人民医院检验科,河北石家庄050051

出　　处：《中国老年学杂志》2012年第19期4191-4193,共3页Chinese Journal of Gerontology

基　　金：河北省卫生厅重点课题(20100193)

摘　　要：目的检测高饱和脂肪酸饮食诱导胰岛素抵抗大鼠肝脏和脂肪胰岛素信号表达改变,探讨高脂肪酸饮食诱导胰岛素抵抗的发病机制。方法将大鼠分为普通饲料对照组(N组)和饱和脂肪酸组(S组);酶免法测定血中胰岛素水平,IGTT实验确定胰岛素抵抗形成,计算胰岛素抵抗指数(IRS)实时PCR检测肝脏和脂肪组织IRS-1,PI3K,Akt,GLUT4基因表达改变。结果相同条件喂养,饱和脂肪酸饮食和普通饲料对大鼠体重和空腹血糖无明显差异;饱和脂肪酸组大鼠血浆胰岛素水平上升,IRS和葡萄糖曲线下面积高于对照组(P<0.05);饱和脂肪酸组大鼠肝脏组织和腹部脂肪组织IRS-1,PI3K,Akt,GLUT4基因表达较对照组明显下降(P<0.05)。结论高饱和脂肪酸饮食引起大鼠血糖正常,胰岛素含量上升,但是肝脏和脂肪胰岛素信号通路基因表达下调,是高饱和脂肪酸饮食诱导大鼠胰岛素抵抗的重要发病机制。Objective To detect the changes of insulin signal expression in liver and fat of rats of insulin resistance induced by high saturated fatty acid （HSFA） diet, then discuss about pathogenesis of insulin resistance caused by HSFA. Methods The rats were divided into control （ N Group） and saturated fatty acid groups （ S Group）. The level of insulin in blood was measured through enzyme immunoassay. Intravenous glucose tolerance test （IGTT） of rats was observed to determine the model of insulin resistance. The changes of gene expression of IRS-1, PI3K, Akt, GLUT4 in liver and fat tissue were detected by real-time PCR. Results In same feed conditions, saturated fatty acid diet and normal forage had no obvious influences in rats＇ mass and fasting blood sugar. In saturated fatty acid group, the level of plasma insu- lin of rats was increased, insulin resistance index （IRS） and insulin in the area of the curve were higher than those in control group （P 〉 0.05）. The down-regulated expression of IRS-1, PI3K, Akt, GLUT4 in rat＇s liver and visceral fat tissue in saturated fatty acid group were measured. Conclusions High saturated fatty acid diet couldn＇t increase changes in rat＇s blood sugar but ascend content of insulin, however the decrease of gene representation in liver and insulin in fat＇s signaling pathway might be the main pathogenesis of high saturated fatty acid leading rats insulin resistance.

关 键 词：饱和脂肪酸 胰岛素抵抗 胰岛素受体 信号事件 代谢综合征 

分 类 号：R587.1[医药卫生—内分泌]