氨氯地平经核因子κB下调氧化型低密度脂蛋白诱导的人脐静脉内皮细胞血小板源生长因子B的表达  

Amlodipine Down-Regulates Platelet-Derived Growth Factor-B Expression in HUVEC-12 Induced by Oxidized Low Density Lipoprotein Through Nuclear Factor-κB

作　　者：卢琼[1,2] 何谨[1,3] 周志刚[1,3] 孟军[1,3] 涂剑[4] 涂玉林[1] 

机构地区：[1]南华大学医学院,湖南省衡阳市421001 [2]怀化医学高等专科学校,湖南省怀化市418000 [3]南华大学附属第一医院,湖南省衡阳市421001 [4]南华大学药物药理研究所,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2012年第10期895-898,共4页Chinese Journal of Arteriosclerosis

基　　金：湖南省卫生厅科技项目(B2007101);博士启动基金(B2011XQD44)资助

摘　　要：目的观察氨氯地平对氧化型低密度脂蛋白(ox-LDL)诱导的人脐静脉内皮细胞(HUVEC-12)中血小板源生长因子B(PDGF-B)和核因子κB(NF-κB)表达的影响,通过氨氯地平对ox-LDL/NF-κB/PDGF-B的影响,进一步探讨氨氯地平相关的作用机制。方法不同浓度氨氯地平(0、0.1、1.0、10.0μmol/L)预先处理细胞0.5 h后加入50mg/L ox-LDL共同孵育24 h,RT-PCR和Western Blot检测细胞内PDGF-B的表达及NF-κB的蛋白表达;进一步通过NF-κB抑制剂吡咯烷二硫代氨基甲酸盐(PDTC)的加入,RT-PCR和Western blot观察其对PDGF-B及NF-κB表达的影响。结果随着氨氯地平处理浓度的增加,HUVEC-12中PDGF-B的mRNA和蛋白表达,以及NF-κB的蛋白表达均明显下调,NF-κB抑制剂PDTC既可降低NF-κB的蛋白表达,也可下调PDGF-B的表达,作用与10.0μmol/L氨氯地平组的结果相近。结论氨氯地平可通过NF-κB下调ox-LDL诱导的HUVEC-12中PDGF-B的表达。Aim To observe the effect of Amlodipine on the expression of platelet-derived growth factor-B （PDGF-B） and nuclear factor-κB （NF-κB） in human umbilical vein endothelial cell （HUVEC-12） induced by oxidized low density lipoprotein （ ox-LDL）, further to explore the related mechanism of Amlodipine through its role on ox-LDL/NF- κB/PDGF-B. Methods After pretreated with different concentration （0, 0. 1, 1.0, 10. 0 μmol/L） of Amlodipine for 0. 5 h and treated with 50 mg/L ox-LDL for 24 h, the expressions of PDGF-B and NF-κB protein were respectively detected by reverse transcription-polymerase chain reaction （RT-PCR） and Western Blot. Then, Pyrrolidinedithiocarbamic acid, ammonium salt（ PDTC）, a kind of NF-κB inhibitor was added, and RT-PCR and Western Blot were used to detect the ex- pression of PDGF-B and NF-κB. Results With the increasing concentration of Amlodipine, the PI）GF-B and NF-κB protein expressions were all down-regulated obviously. PDTC not only down-regulated the NF-κB protein expression, but also the PDGF-B expression, as effectively as 10. 0 μmol/L Amlodipin group. Conclusion Amlodipine can down-reg- ulate the PDGF-B expression in HUVEC-12 induced by ox-LDL through NF-κB.

关 键 词：氨氯地平 氧化型低密度脂蛋白 人脐静脉内皮细胞 核因子ΚB 血小板源生长因子B 

分 类 号：R363[医药卫生—病理学]