Period1、Period2对胶质瘤细胞的生物节律性调控作用  

Regulatory effect of Period1 and Period2 on biological rhythm in glioma cells

作　　者：李延辉[1] 王凡[2] 尹磊[1] 李张珂[1] 牛占峰[3] 夏鹤春[3] 

机构地区：[1]宁夏医科大学,750004 [2]荆门市第一人民医院神经外科 [3]宁夏医科大学总院神经外科

出　　处：《江苏医药》2012年第18期2109-2112,共4页Jiangsu Medical Journal

基　　金：国家自然科学基金(30860289);宁夏医科大学科研基金(XM201039)

摘　　要：目的研究大鼠胶质瘤细胞内生物钟基因Period1(Per1)、Per2mRNA及蛋白的周期表达规律。方法将体外培养的大鼠胶质瘤C6细胞接种于SD大鼠右侧尾状核区,在12h光照和12h黑暗的标准昼夜节律环境下,采用RT-PCR和免疫组织化学方法分别检测成瘤后4、8、12、16、20、24h肿瘤组织中Per1、Per2 mRNA和蛋白的表达。结果在大鼠胶质瘤组织中,Per1、Per2mRNA及蛋白产物呈节律性表达,其表达周期为12h的超日节律。Per1mRNA和蛋白的高表达时间点均位于4、16h,低表达时间点位于12、24h;Per2mRNA和蛋白的高表达时间点位于12、24h,低表达时间点位于8、20h。结论在SD大鼠体内,胶质瘤细胞的Per1、Per2基因呈12h的超日节律表达,提示生物钟基因的表达异常与胶质瘤的发生发展具有相关性。Objective To investigate the periodical regularity of mRNA and protein expressions of bioclock gene Periodl （Perl） and Per2 in rat glioma cells. Methods The C6 glioma cells were cultured in vitro and inoculated into the right caudal nucleus of sixty SD rats. And then the rats were bred under the circumstance of a standardized 12 h light and 12 h dark cycle. At 4,8,12,16,20,24 h after tumor formation, the rnRNA and protein expressions of Per2 and Per2 were detected by RT-PCR and immunohistochemistry, respectively. Results The mRNA and protein expressions of Per1 and Per2 in glioma tissues showed a 12-h ultradian rhythm. The mRNA and protein expressions of Per1 peaked at 4 and 16 h,which were the lowest at 12 and 24 h. The mRNA and protein expressions of Per2 peaked at 12 and 24 h,which were the lowest at 8 and 20 h. Conclusion The expressions of Perl and Per2 in the glioma tissues of SD rats present a 12-h ultradian rhythm, which suggests that the development of glioma may he correlated with the abnormal expression of hioclock gene.

关 键 词：Period1 Period2 胶质瘤 生物节律性 

分 类 号：R73[医药卫生—肿瘤]