己烯雌酚对HOSE细胞中HOXA10基因表达及DNA甲基化的影响  

Effect of diethylstilbestrol on expression and DNA methylation of HOXA10 gene in HOSE cell line

作　　者：万一聪[1] 张林[1] 姜旖[2] 马小玲[1] 程文俊[2] 

机构地区：[1]南京医科大学第一临床医学院,江苏省210029 [2]南京医科大学第一附属医院妇科

出　　处：《江苏医药》2012年第18期2113-2115,共3页Jiangsu Medical Journal

基　　金：江苏省科技厅自然科学基金(BK2010576);江苏省六大人才高峰(303070774IB09)

摘　　要：目的研究己烯雌酚对人正常卵巢上皮细胞株HOSE中HOXA10基因表达及基因启动子区CpG岛甲基化的影响。方法用不同浓度的己烯雌酚(5×10-6、5×10-7、5×10-8 mol/L)作用于体外培养的人正常卵巢上皮细胞株HOSE。培养5d后收集细胞,应用实时荧光定量PCR和Western blot检测HOXA10基因mRNA和蛋白的表达;运用甲基化特异性PCR检测HOXA10基因启动子区CpG岛的甲基化状态。结果己烯雌酚作用后,HOSE细胞中HOXA10基因mRNA和蛋白表达上调(P<0.05),并且HOXA10基因启动子区发生去甲基化改变。结论己烯雌酚可以上调人正常卵巢上皮细胞株HOSE中HOXA10基因的表达,与其对HOXA10基因启动子区CpG岛甲基化的影响有关。Objective To study the effect of diethylstilbestrol （DES） on the expression and promoter region CpG islands methylation of HOXA10 gene in human normal ovarian epithelial cell line HOSE. Methods The human normal ovarian epithelial cell line HOSE was cultured in vitro with DES in different concentrations（5 × 10-6 , 5 ×10-7 and 5× 10-8 tool/L） for 5 days. RT-PCR and Western blot were used to detect the expressions of HOXA10 mRNA and protein. The methylation specific polymerase chain reaction（MSP） was used to detect HOXA10 gene promoter region CpG islands methylation status. Results After dosed with DES, the HOXA10 mRNA and protein expressions were upregulated （P〈0. 05 ）, and the HOXA10 gene promoter region CpG islands showed demethylation. Conclusion DES can upregulate HOXA10 expression in human normal ovarian epithelial cell line HOSE and this is associated with the change in the methylation of HOXA10 gene promoter region CpG islands induced by DES.

关 键 词：己烯雌酚 卵巢上皮性癌 同源盒基因 DNA甲基化 

分 类 号：R737[医药卫生—肿瘤]