大鼠玻璃体内注射尿激酶对视网膜血管通透性的影响  

Effects of intravitreal injecting urokinase on retinal vascular permeability of rats

作　　者：刘会荣[1] 高永峰[1] 

机构地区：[1]郑州大学第一附属医院眼科,河南省郑州市450052

出　　处：《眼科新进展》2012年第10期939-941,共3页Recent Advances in Ophthalmology

摘　　要：目的观察大鼠玻璃体内注射尿激酶(urokinase,UK)对视网膜血管通透性的影响。方法 SD大鼠30只,随机分为2组,每组15只(15眼)。实验组大鼠右眼玻璃体内注射UK,对照组大鼠右眼玻璃体内注射磷酸盐缓冲液。24h后大鼠尾静脉注射追踪剂依文思蓝(evansblue,EB),2h后摘出大鼠右眼球,取出玻璃体,使用间接酶联免疫吸附试验(ELISA)检测玻璃体内EB吸光度。测定绘制EB浓度-吸光度的标准曲线,计算大鼠玻璃体内EB的质量浓度。结果 EB的浓度在0～30mg·L-1时,吸光度为0.0080～0.1200,线性关系良好,回归方程为y=0.0034x+0.0044(r2=0.9941)。EB注射后摘取大鼠眼球,观察眼球的色泽变化:实验组为深蓝色,对照组为无色透明或浅蓝色。间接酶联免疫吸附试验结果显示:实验组EB吸光度值为0.0558±0.0112,对照组为0.0145±0.0077,差异有统计学意义(t=11.746,P<0.05)。由EB浓度-吸光度标准曲线得出实验组大鼠玻璃体内EB质量浓度为(0.6043±0.1316)g·L-1,对照组为(0.1324±0.0865)g·L-1,差异有统计学意义(t=11.607,P<0.05)。结论大鼠玻璃体内注射UK可以增加视网膜血管的通透性。EB可以作为一种测量视网膜血管通透性的理想追踪剂。Objective To research the effects of intravitreal injecting urokinas （UK） on retinal vascular permeability of rats.Methods Thirty healthy SD rats were randomly divided into two groups,15 cases in each group.UK was intravitreally injected in right eye for experimental group and PBS for control group.All the rats were injected tracer evans blue （EB） via the caudal vein after 24 hours.After 2 hours,all the vitreous of right eyes were removed,EB absorbance in vitreous was measured by the indirect enzyme-linked immunosorbent assay （ELISA）.EB concentration-absorbance relationship standard curve was obtained,and the concentration of EB in vitreous was calculated.Results When the concentration of EB was from 0 to 30 mg·L-1,its absorbance was from 0.008 0 to 0.120 0,and it had a good liner relationship according to the concentration-absorbance standard curve of EB,the regression equation was y=0.003 4x＋0.004 4（r2=0.994 1）.The reflected color of eyeball in the experimental group after injecting EB was dark blue,while the control group was colorless or light blue.The average absorbance of EB in vitreous measured by indirect ELISA was 0.055 8±0.011 2 in the experimental group and 0.014 5±0.007 7 in the control group,there was statistical difference between two groups（t=11.746,P0.05）.The average concentration of EB in vitreous according to the EB concentration-absorbance relationship standard curve was（0.604 3±0.131 6）g·L-1 in the experimental group and （0.132 4±0.086 5）g·L-1 in the control group,there was statistical difference two groups（t=11.607,P0.05）.Conclusion Intravitreal injecting UK can increase the retinal vascular permeability of rats.EB can be set as a sensitive and quantitative tracer in measuring retinal vascular permeability.

关 键 词：尿激酶 依文思蓝 间接酶联免疫吸附试验 

分 类 号：R774.1[医药卫生—眼科]