不同光源共焦显微镜的活体正常角膜图像研究  

The in vivo study of different light sources confocal microscopy in normal cornea

作　　者：黎明[1] 姚晓明[1] 林跃生[2] 于莉[1] 林宝涛[1] 张醇[1] 聂丹瑶[1] 

机构地区：[1]暨南大学附属深圳市眼科医院 , 广东深圳518040 [2]中山大学中山眼科中心

出　　处：《中国实用眼科杂志》2012年第10期1176-1182,共7页Chinese Journal of Practical Ophthalmology

摘　　要：目的分别采用卤素灯光源共焦显微镜和激光光源共焦显微镜对中国正常成人活体角膜各层组织结构进行观察，对照研究其图像特征。方法对35例（70只眼）中国成人（年龄18。55岁）中央部角膜分别采用卤素灯光源共焦显微镜和激光光源共焦显微镜进行检查，研究角膜各层结构的图像特点，并进行对比。结果共焦显微镜检查的35例（70只眼）中，67只眼95．7％成功获得了角膜上皮翼状细胞层的图像，70只眼100％成功获得角膜上皮基底细胞层、前弹力层、前基质层、后基质层及角膜内皮细胞层的图像。结论卤素灯光源共焦显微镜的放大倍数是1000倍，细胞放大倍数大，近似于初级电镜的放大倍数，但卤素灯光源的组织穿透力较弱，光源易衰减，所得的角膜各层细胞图像较激光角膜共焦显微镜的略微模糊，所得角膜各层图像总体颜色偏灰、结构偏模糊。激光角膜共焦显微镜的放大倍数是800倍，较卤素灯光源的共焦显微镜略小，但激光光源的组织穿透力强，所得角膜各层细胞图像较卤素灯光源的更清晰，而且在特定角度可获得非常类似角膜组织病理学切片的图像。Objective To observe the different organizational structures of corneal layers of nor- mal Chinese adult by halogen light source confocal microscopy and laser light source confocal mi- croscopy in vivo, to study and compare its image features. Methods The central parts of the cor- nea were examined by halogen light source confocal microscopy and laser light source confocal mi- croscopy from 35 cases （70 eyes） adult （age from 18 to 55 years old）. The image characteristics of organizational structures of different corneal layers were studied and compared. .Results The corneal epithelial wing-like cell layer images of 67 eyes （95.7%） were successfully examined. The basal epi- thelium, Bowman＇s membrane, anterior stroma, posterior stroma, Dscemet＇s membrane, endothelium were successful examined 100%. Conclusions The magnification of halogen light confocal microsco- py are 1000 times, and the better magnification of cells. It＇s similar to the magnification of the pri- mary electron microscopy. Because of the weak tissue penetration of the halogen light source and easy to decay, the cell images of different corneal layers of halogen light confocal microscopy are more blurred than laser comeal confocal microscopy, and the color of that are more gray, the struc- ture of that are partial fuzzy; The magnification of laser corneal confocal microscopy are 800 times, cell magnification of that are slightly smaller than that of halogen light confocal microscopy. The cell images of different corneal layers of laser corneal confocal microscopy are more clearly than that of halogen light confocal microscopy, because of the stronger tissue penetration of the laser light source. And the very similar to the corneal tissue pathology slice images are available at a par-ticular point of view.

关 键 词：共焦显微镜 角膜 卤素灯 激光 

分 类 号：R772.21[医药卫生—眼科]