Exploration on Separation of Periplaneta Americana Small Peptides with Tricine-SDS-PAGE System  

作　　者：Shaohui WU Xiumei WU Chenggui ZHANG Guangmin DONG Guangming LIU 

机构地区：[1]College of Pharmacy, Dali University

出　　处：《Agricultural Biotechnology》2012年第2期14-16,共3页农业生物技术（英文版）

基　　金：Supported by National Natural Science Foundation of China ( 30560181);Key Industry Innovation Project of Yunnan Province ( 2008IF012)

摘　　要：[ Objective] This study aimed to explore a Tricine-SDS-PAGE method for separation and purification of Periplaneta Americana small peptides. [ Method] By comparing the separation effects of three types of gels with different ratios on P. Americana small peptides, an appropriate Tricine-SDS-PAGE method for separation of small peptides was established. [ Result] Standard low molecular weight Marker was preliminarily separated to a certain extent with polyacrylamide gel electraphoresis, while the sample mixture was not separated due to the different levels of aggregation in separating gel, spacer gel and stacking gel. [Condusion] More appropriate polyacrylamide gel electrephoresis separation method or other better separation methods were to be further explored for separating peptide mixture with smaller molecular weight.[ Objective] This study aimed to explore a Tricine-SDS-PAGE method for separation and purification of Periplaneta Americana small peptides. [ Method] By comparing the separation effects of three types of gels with different ratios on P. Americana small peptides, an appropriate Tricine-SDS-PAGE method for separation of small peptides was established. [ Result] Standard low molecular weight Marker was preliminarily separated to a certain extent with polyacrylamide gel electraphoresis, while the sample mixture was not separated due to the different levels of aggregation in separating gel, spacer gel and stacking gel. [Condusion] More appropriate polyacrylamide gel electrephoresis separation method or other better separation methods were to be further explored for separating peptide mixture with smaller molecular weight.

关 键 词：Periplaneta Americana HPLC method SDS-PAGE 

分 类 号：R284[医药卫生—中药学]