Selection of Phage-resistant Strains from Escherichia coli glyA Genetic Engineering Bacteria  

作　　者：Yunjun MEI Hong LIU 

机构地区：[1]School of Chemical and Environmental Engineering, Wuhan Polytechnic University [2]Three Gorges High School

出　　处：《Agricultural Biotechnology》2012年第2期35-37,共3页农业生物技术（英文版）

摘　　要：[ Objective] This study aimed to select E. coli strains with phage-resistance. [ Method] Phage-resistant strains of E. coli glyA genetic engineering bacteria were selected by phage induction and UV-coupling phage induction. [ Result] By phage induction, 20 strains with stable resistance were selected from the 24 phage-resistant strains, only one strain showed better growth condition than the original strains, but the enzymatic activities of the 20 strains were all lower than the original strains; 41 phage-resistant strains were selected by UV-coupling phage induction, 39 strains of which had better stability, including 7 strains that showed better growth conditions than the original strains and two strains had higher enzymatic activities than the original strains. [ Conclusion] UV-coupling phage induction is a suitable method to select phage-resistant strains from Ecoli genetic engineering bacteria.[ Objective] This study aimed to select E. coli strains with phage-resistance. [ Method] Phage-resistant strains of E. coli glyA genetic engineering bacteria were selected by phage induction and UV-coupling phage induction. [ Result] By phage induction, 20 strains with stable resistance were selected from the 24 phage-resistant strains, only one strain showed better growth condition than the original strains, but the enzymatic activities of the 20 strains were all lower than the original strains; 41 phage-resistant strains were selected by UV-coupling phage induction, 39 strains of which had better stability, including 7 strains that showed better growth conditions than the original strains and two strains had higher enzymatic activities than the original strains. [ Conclusion] UV-coupling phage induction is a suitable method to select phage-resistant strains from Ecoli genetic engineering bacteria.

关 键 词：Escherichia coli PHAGE phage-resistant strains SELECTION 

分 类 号：R392[医药卫生—免疫学]