PSMB4亚基蛋白在脑胶质瘤中表达的相关研究  

Expression of proteasome subunit beta type-4 protein in gliomas

作　　者：谭国伟[1,2] 陈建彬[1] 钱洪流[3] 王占祥[2] 陶涛[1] 

机构地区：[1]厦门大学生命科学学院,厦门361005 [2]厦门大学附属第一医院神经外科,厦门361003 [3]厦门大学医学院病理学教研室,厦门361005

出　　处：《中华神经医学杂志》2012年第10期1015-1018,共4页Chinese Journal of Neuromedicine

摘　　要：目的 探讨26S蛋白酶体的PSMB4亚基（PSMB4）在正常脑组织与胶质瘤组织中表达的差异性及其与脑胶质瘤恶性程度的相关性。方法 选取厦门大学附属第一医院神经外科自2008年1月至2012年2月问手术切除并经病理证实的人脑胶质瘤液氮冻存标本75例，其中星形细胞瘤Ⅰ级21例，星形细胞瘤Ⅱ级21例，星形细胞瘤Ⅲ级27例，胶质母细胞瘤Ⅳ级6例。另取5例因脑创伤行内减压术患者的正常脑组织液氮冻存标本，免疫组织化学染色检测标本中PSMB4的表达。结果 PSMB4在正常脑组织中呈阴性表达，而在胶质瘤组织中呈阳性或强阳性表达。统计分析显示胶质瘤组PSMB4蛋白的表达明显高于正常脑组织组，差异有统计学意义（P〈0．05），不同级别胶质瘤PSMB4表达的分布差异无统计学意义（P〉0．05）。结论 脑胶质瘤组织PSMB4蛋白的表达高于正常脑组织，但与脑胶质瘤的恶性程度无明显相关性。Objective To investigate the differences of proteasome subunit beta type-4 （PSMB4） expression between normal cerebral tissues and glioma tissues, and the relevance between its expression and glioma malignancy degree. Methods Seventy-five human glioma liquid nitrogen frozen specimens, collected and confirmed by pathology in our hospital from January 2008 to February 2012, including 21 with grade I astrocytoma, 21 with grade II astrocytoma, 27 with grade III astrocytoma and 6 with grade IV glioblastoma, were chosen in our study; another 5 normal cerebral tissues from cerebral trauma patients performed decompression were chosen as controls. Immunohistochemistry wax employed to detect the PSMB4 expressions in both normal cerebral tissues and glioma tissues. Results PSMB4 protein expression was negative in the controls, while positive/strong positive in the gliomas. Statistical analysis showed that the PSMB4 protein expression level in the gliomas was significantly higher than that in the controls （P〈0.05）. PSMB4 protein expression in gliomas of different degrees showed no statistically significant differences （P〉0.05）. Conclusion PSMB4 protein expression in the glioma tissues is higher than that in the normal cerebral tissues, however, no relevance between PSMB4 expression and glioma malignancy grade is noted.

关 键 词：神经胶质瘤 PSMB4 免疫组化染色 

分 类 号：R739.4[医药卫生—肿瘤]