低氘水可选择性抑制鼻咽癌细胞增殖  

Deuterium-depleted water selectively inhibits nasopharyngeal carcinoma cell proliferation in vitro

作　　者：王宏强[1,2] 祝葆华[1] 刘聪[2] 方唯意[3] 杨慧龄[1,2] 

机构地区：[1]广东医学院中美肿瘤研究所,广东东莞523808 [2]南华大学药物药理研究所,湖南衡阳421001 [3]南方医科大学基础医学院肿瘤研究所,广东广州510515

出　　处：《南方医科大学学报》2012年第10期1394-1399,共6页Journal of Southern Medical University

基　　金：国家自然科学基金(30971438;81272334)~~

摘　　要：目的比较不同氘体积浓度对多种鼻咽癌细胞株及正常细胞增殖的作用,初步探讨低氘水抗肿瘤的可能机制。方法 MTT法、平皿克隆实验、transwell小室检测低氘水(DDW)对鼻咽癌细胞及正常细胞的增殖、侵袭转移能力;Western blot检测PCNA蛋白表达差异,流式细胞仪分析细胞周期。结果随着培养基中氘含量下降,多种鼻咽癌细胞增殖显著被抑制(P<0.05),克隆生成和游走侵袭能力显著下降(P<0.01),Western blot检测结果显示PCNA蛋白表达水平下调,流式细胞仪检测显示,低氘水能诱导鼻咽癌细胞生长周期阻滞:S期减少,G1期细胞显著增加(P<0.05)。但DDW对正常细胞生长有促进作用。结论 DDW具有选择性抑制鼻咽癌细胞增殖的靶向生物效应,因此其可作为新型无毒副作用抗癌辅助治疗剂,在肿瘤治疗中具有重要应用前景。Objective To evaluate the inhibitory effect of deuterium-depleted water（DDW） on the proliferation of nasopharyngeal carcinoma（NPC） cells in vitro and explore the possible mechanism.Methods The growth inhibition of NPC cells and preosteoblast MC3T3-E1 cells following DDW treatment was measured by MTT assay and plate colony formation assay.The changes in migration and invasion of NPC cells were evaluated using Transwell and boyden chamber assays.The protein expression of proliferating cell nuclear antigen（PCNA） was determined using Western blotting.Flow cytometry was employed to evaluate the changes in cell cycle distribution after DDW treatment.Results DDW with deuterium concentrations of 100,75 and 50 ppm significantly suppressed the cell proliferation（P0.05） and lowered colony formation capacity and invasiveness of the NPC cells（P0.01）.Western blotting demonstrated a down-regulated expression of PCNA in the cells by DDW.DDW also caused obvious cell cycle arrest in the NPC cells with reduced cells in S phase and significantly increased cells in G1 phase（P0.05）.Rather than causing growth inhibition,DDW promoted the growth of normal control MC3T3-E1 cells.Conclusion DDW possesses selective biological effects to inhibit the proliferation of NPC cells in vitro,suggesting the potential of DDW as a novel nontoxic adjuvant therapeutic agent in antitumor therapy.

关 键 词：低氘水 鼻咽癌细胞 前成骨细胞 增殖 PCNA蛋白 

分 类 号：R739.63[医药卫生—肿瘤]