HSV-2 LAT过表达vero细胞中microRNA的表达谱变化  

Changes of microRNA expression profiles in Vero cells induced by HSV-2 LAT overexpression

作　　者：王颖[1] 樊建勇[1] 杨慧兰[1] 陈剑云 

机构地区：[1]广州军区广州总医院皮肤科 [2]福建省73881部队卫生处

出　　处：《南方医科大学学报》2012年第10期1440-1444,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(81071401);广东省科技计划(2009B080701091)~~

摘　　要：目的分析过表达疱疹病毒Ⅱ型(HSV-2)潜伏相关转录子LAT后vero细胞microRNA表达谱的表达变化。方法通过化学合成的方法获得LAT基因的全长序列。构建HSV-2 LAT逆转录病毒表达载体pRetroQ-AcGFP1-C1,包装获得HSV-2 LAT基因过表达的逆转录病毒。利用microRNA芯片技术,分析感染逆转录病毒HSV-2 LAT后vero细胞microRNA表达谱的变化,得到因LAT基因过表达而产生变化的microRNA。结果通过microRNA芯片分析,逆转录病毒HSV-2 LAT感染vero细胞后,可引起hsa-miR-23a*、kshv-miR-K12-3、hsa-miR-943、hsa-miR-634、hsa-miR-1270 5种microRNA2倍以上上调;使hsa-miR-181a-2*、hsa-miR-450b-5p、hsa-miR-31、hsa-miR-24、kshv-miR-K12-12*5种microRNA2倍以上下调。结论 LAT基因过表达后可引起vero细胞microRNA表达谱的变化。Objective To investigate the changes in the microRNA expression profile of Vero cells induced by HSV-2 LAT overexpression.Methods The full-length open reading frame of HSV-2 LAT was synthesized and cloned into pRetroQ-AcGFP1-C1 vector,and the recombinant retrovirus expressing HSV-2 LAT was packaged.Using a microRNA microarray,the microRNA expression profile changes in Vero cells were analyzed after infection with the recombinant retrovirus.Results In Vero cells infected with the recombinant retrovirus for stable HSV-2 LAT overexpression,5 microRNAs（hsa-miR-23a＊,kshv-miR-K12-3,hsa-miR-943,hsa-miR-634,and hsa-miR-1270） were up-regulated and 5（hsa-miR-181a-2＊,hsa-miR-450b-5p,hsa-miR-31,hsa-miR-24,and kshv-miR-K12-12＊） were down-regulated.Conclusion The expression of HSV-2 LAT can induce changes in microRNA expression profile in Vero cells.

关 键 词：疱疹病毒Ⅱ型 LAT MICRORNA 基因芯片 逆转录病毒 

分 类 号：R363[医药卫生—病理学]