荧光原位杂交技术在膀胱尿路上皮癌的诊断和复发中的应用  

Application of fluorescent in situ hybridization in the diagnosis and recurrence of bladder cancer

作　　者：刘亚东[1] 顾沈阳[1] 王业华[1] 吴蔚[1] 

机构地区：[1]苏北人民医院泌尿外科,江苏扬州225001

出　　处：《临床泌尿外科杂志》2012年第10期770-773,共4页Journal of Clinical Urology

摘　　要：目的:探讨荧光原位杂交技术(fluorescent in situ hybridazation,FISH)在膀胱尿路上皮肿瘤的诊断和术后复发中的应用。方法:选取2008年8月～2011年12月67例膀胱肿瘤患者,包括10例非尿路上皮癌患者和57例膀胱尿路上癌患者,留取受检者的晨尿,做FISH检测。结果:FISH检测初发膀胱肿瘤患者的敏感性是81.3%,FISH检测复发膀胱肿瘤的敏感性是88.9%,FISH检测初发膀胱肿瘤和复发膀胱肿瘤的敏感性差异无统计学意义。随着肿瘤分级的增高,FISH检测的总敏感性逐渐增高(G179.2%、G281.0%、G391.7%),但中低级肿瘤与高级别肿瘤的敏感性差异无统计学意义(χ2=0.267,P=0.605>0.05)。FISH在检测肿瘤个数为1个、2～7个、>7个时的总敏感性分别是80.9%、88.9%、100%。FISH在检测肿瘤直径<3cm、≥3cm时总的敏感性分别是80.0%、91.7%。结论:FISH在检测初发和复发膀胱肿瘤的敏感性均较高,所以FISH技术作为诊断和检测肿瘤复发的手段,能明显提高检测肿瘤的效率。Objective：To explore the application of fluorescent in situ hybridization（FISH）in the diagnosis and recurrence of urothelial tumor of bladder. Method： From August 2008 to December 2011,67 volunteers with informed consent were divided into three groups. In these groups, 10 noneurothelial tumor of bladder individuals as control,48 patients were proven as urothlial tumor of bladder by pathological,and 9 patients were proven as recurrence of bladder tumor by pathological. Urine were collected and tested by FISH. Result：The sensitivity of FISH was 81.3% in initial bladder cancer and that of the recurrence bladder cancer was 88.9%. while The sensitivity of FISH in different grades（G1 , G2 , G3 ）was 79.2% ,81.0% ,91.7%. Overall sensitivity of bladder carcinoma between G1, G2 and Ga hasn＇t significant difference. The sensitivity of FISH in detecting the different number of tumors（ 1, 2-- 7, 〉7） was 80.9 %, 88.9 %, 100.0 %. The sensitivity of FISH in detecting different diameters （ 〈 3 cm, ≥3 cm）was 80.0 %,91.7 %. Conclusion.. FISH as a newly non-invasive technique has a high sensitivity in ini- tial and recurrence bladder cancer . FISH may play an important role in improving the rate of bladder cancer.

关 键 词：膀胱肿瘤 荧光原位杂交技术(FISH) 肿瘤复发 敏感性 

分 类 号：R737.14[医药卫生—肿瘤]