低声压级次声对小鼠海马胶质纤维酸性蛋白表达的影响  

The effect of infrasound of low sound pressure level on the expression of glial fibrillary acidic protein in hippocampus of mice

作　　者：彭丽岚[1] 牟翔[2] 袁华[2] 李玲[3] 唐晨[1] 张斐[1] 王方聚[1] 赵联伟[1] 陈雷[1] 

机构地区：[1]解放军济南军区烟台疗养院理疗科,烟台264001 [2]第四军医大学西京医院康复与理疗科 [3]解放军总医院第一附属医院康复理疗科

出　　处：《中国康复医学杂志》2012年第10期899-902,共4页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金项目(30970674)

摘　　要：目的:研究低声压级次声作用不同时间对小鼠海马胶质纤维酸性蛋白(GFAP)表达的影响。方法:将60只雄性BALB/c小鼠随机分为对照组和次声作用组,次声作用组以Infratonic9次声治疗仪产生的次声(频率为8—12Hz,声强为60—80dB)作用,1h/d,分别作用1d、7d、14d、21d、28d,对照组小鼠除无次声作用,其余处理皆与次声作用组相同。各组小鼠实验结束后取脑,采用免疫荧光化学染色方法观察次声作用不同次数(1d、7d、14d、21d、28d)小鼠海马中GFAP的表达情况。结果:次声作用1d后小鼠海马中GFAP阳性表达无明显变化(62.9±3.0),7d后GFAP阳性细胞数开始减少(60.5±8.0),连续作用21d达到最低值(56.3±5.3()P<0.05),28d回升至正常水平(59.2±9.7()P>0.05)。结论:次声治疗仪产生的低声压级次声作用能抑制小鼠海马星形胶质细胞的活化,这为次声的临床治疗提供了理论依据。Objective：To study the expression of glial fibrillary acidic protein（GFAP） in hippocampus of mice after exposure to iafrasound of low sound pressure level for different times. Method： Sixty BALB/C male mice were randomized into control group and infrasound exposure group. Mice of the infrasound exposure group were exposed to infrasound of low sound pressure level （8--12Hz, 60---$OdB） generated by infratonic 9 instrument, lh/d forl,7,14,21 and 28 days. Mice of control group were treated as the infrasound group except for the infrasound exposure. Brains of mice were removed and immunofluorescent staining method was used to detect the expression of GFAP in hippocampus right after the treatment. Result： The expression of GFAP-positive cells in hippocampus showed after once exposure there was no signifi- cant change （62.9 ± 3.0）, after exposure for 7 days decreased（60.5 ± 8.0）, after exposure for 21 days reached to the least（56.3 ± 5.3）（P〈0.05）, after exposure for 28 days increased to normal level（59.2± 9.7）（P〉0.05）. Conclusion： Infrasound of low sound pressure level generated by infrasound instrument could inhibit the activation of GFAP-positive astrocytes in hippocampus of mice, which might provide the theory basis for the clinical thera- py of infrasound.

关 键 词：次声 小鼠 海马 胶质纤维酸性蛋白 

分 类 号：R363[医药卫生—病理学]