CDX2基因在初发急性髓系白血病患者中的表达及其临床意义  

Expression level of CDX2 gene in acute myeloid leukemia and its clinical significance

作　　者：陆滢[1] 汪琼[2] 牧启田 余梦霞[1] 陈志妹[2] 楼基余[2] 金洁[4] 

机构地区：[1]浙江省宁波市第一医院血液科,315000 [2]浙江省宁波市宁波大学 [3]浙江省杭州市血液病研究所 [4]浙江省杭州市浙江大学附属第一医院血液科

出　　处：《中华血液学杂志》2012年第10期835-838,共4页Chinese Journal of Hematology

摘　　要：目的研究同源框转录因子2（Caudal-typehomeoboxtranscriptionfactor2，CDX2）基因在急性髓系白血病（AML）患者中的表达及其临床意义。方法采用实时定量PCR（RQ—PCR）方法检测108例初发AML患者骨髓单个核细胞CDX2基因的表达，并以7名健康人骨髓作为对照分析其临床意义。结果108例AML患者的骨髓单个核细胞均检测到CDX2基因表达，中位表达水平为1179．44（14．15～867961．10）。以第1个四分位数为界分为低表达组和高表达组，低表达组27例，高表达组81例。7名健康对照者骨髓单个核细胞也均检测到CDX2表达，但表达水平较低，中位表达水平为105．30（22．30—453．11），健康对照与AML患者之间CDX2基因表达差异有统计学意义（P〈0．01）；14例FLT3-ITD＋患者均在CDX2高表达组（P=0．018），其中10例为正常核型。在接受治疗的83例AML患者和57例WBC≥10×10＋／L的AML患者组中，CDX2高表达患者完全缓解（CR）率均低于CDX2基因低表达患者（54．7％对63．2％，P=0．046；51．1％对75．0％，P=0．048）。结论CDX2基因异常表达与FLT3-ITD基因突变关系密切，并且CDX2基因高表达患者CR率较低，可能为正常核型AML患者预后不良指标。Objective To explore the expression and clinical significance of Caudal-type homeobox transcription factor 2 （CDX2） gene in acute myeloid leukemia （AML） patients. Method Real time quantitative PCR（RQ-PCR） was used to test the expression level of CDX2 gene in 108 de novo AML patients and the clinical features of these patients were analyzed. Results CDX2 gene transcript levels were detectable in bone marrow mononuclear ceils from 108 AML patients and 7 healthy donors, the median expression level were 1179.44 （range 14.15 -867 961.10） and 105.30 （range 22.30 -453.11 ）. There was a statistically significant difference in expression level of CDX2 gene between the AML patients and normal donor （P 〈 0.01 ）. All 14 patients with FL]B-ITD＋ were in CDX2 gene higher expression group （P =0.018）, including 10 patients with normal karyotype. In the 83 treated AML patients （P = 0. 046） and 57 higher WBC count （ ≥ 10 × 10^9/L, P = 0. 048） patients, the higher expression level of CDX2 gene was associated with lower complete remission （CR） rates. Conclusions Higher expression level of CDX2 gene was seen mostly in AML patients with FL＇I3-ITD mutation and with lower CR rates. CDX2 gene might be a prognostic molecular marker in AML patients with normal karyotype.

关 键 词：同源框转录因子 白血病 髓样 急性 预后 

分 类 号：R733.7[医药卫生—肿瘤]