非小细胞肺癌患者外周血Foxp3＋调节性T细胞和Th17的检测与临床意义  

The detection and significance of Foxp3＋ regulatory T cells, Th17 in peripheral blood of non-small cell lung cancer patients

作　　者：李莎[1] 李岩[1] 梁婧[1] 刘晓琳[1] 

机构地区：[1]山东大学附属千佛山医院肿瘤科,济南250014

出　　处：《中华微生物学和免疫学杂志》2012年第7期642-646,共5页Chinese Journal of Microbiology and Immunology

摘　　要：目的通过检测非小细胞肺癌（NSCLC）患者外周血Foxp3＋调节性T细胞（TrFoxp3＋）、Th17细胞、TrFoxp3＋／Th17及相关细胞因子水平，探讨TrFoxp3＋、Th17细胞在肺癌发生发展中的相互作用以及是否存在TrFoxp3＋／Th17失衡。方法采用流式细胞术和酶联免疫吸附法（ELISA）分别检测18例健康人、26例NSCLC患者外周血中TrFoxp3＋、n17细胞占CD4＋T细胞的比例，以及转化生长因子-β（TGF-β）、IL-17、IL-23的表达水平。结果NSCLC患者外周血中TrFoxp3＋、Th17细胞比例、TrFoxp3＋／Th17高于健康对照组（P〈0．05）。NSCLC患者外周血TrFoxp3＋与Th17细胞成正向直线相关的关系（r=0．81，P〈0．05）。不同TNM分期肺癌患者外周血TrFoxp3＋／Th17：Ⅳ期肺癌患者高于Ⅰ-Ⅱ期、Ⅲ期肺癌患者（P〈0．05）。NSCLC患者血清中TGF-β、IL-17、IL-23水平高于健康对照组，TGF-β在晚期组／健康对照组的比值高于早中期组／健康对照组的比值（P〈0．05）。结论NSCLC患者外周血中TrFoxp3＋、Th17细胞比例及TrFoxp3＋／Th17较健康人升高，且晚期患者TrFoxp3＋／Th17明显升高，提示NSCLC患者可能存在TrFoxp3＋／鸭17比例失衡，导致肿瘤患者免疫抑制，促进肿瘤发生、发展，可能与二者的细胞因子调节有关。Objective To detect the Foxp3＋ regulatory T cells （TrFoxp3＋ ）, Th17, TrFoxp3＋/ Th17 and related cell factors level in peripheral blood of non-small cell lung cancer（NSCLC） patients, and to explore the relationships about TrFoxp3＋, Th17 with occurrence and development of lung cancer and whether there are imbalance of TrFoxp3＋/Th17. Methods The proportions of TrFoxp3＋, Th17 cells and the level of related cell factors such as TGF-β, IL-17, IL-23 were determined respectively by flow cytorsetry and ELISA in peripheral blood of 18 healthy people and 26 patients with NSCLC. Results The proportions of TrFoxp3＋, Th17 and TrFoxp3＋/Th17 in peripheral blood with NSCLC were higher than healthy controls（ P 〈0.05 ）. The proportion of Th17 cells from NSCLC patients was positively correlated with that of TrFoxp3 ＋ （ r = 0. 81, P〈0. 05 ）. Comparing TrFoxp3 ＋/Th17 among different TNM stages in NSCLC patients ： TrFoxp3 ＋/ Th17 of in lV stage was obvious higher than that of in Ⅰ - Ⅱstage, Ⅲ stage（P〈0. 05）. The level of TGF-β, IL-17, IL-23 was higher in NSCLC patients than that in healthy controls, the ratio of TGF-β in the advanced stage group/healthy controls was higher in the early and middle group/healthy controls（P〈0.05）. Conclu- sion TrFoxp3＋, Th17 and TrFoxp3 ＋/Th17 in NSCLC patients were higher than healthy people, moreover TrFoxp3＋/Th17 in advanced stage increased significantly. The results may imply that there are certain rela- tionships between the imbalance of TrFoxp3＋/Th17 and immunosuppression and occurrence and development of NSCLC as well as the regulation of their evtokines.

关 键 词：非小细胞肺癌 Foxp3+ 调节性T细胞(Tr) TH17 

分 类 号：R734.2[医药卫生—肿瘤]