结肠癌转移相关基因1mRNA在乙型肝炎病毒相关肝细胞癌中的表达及其临床意义  

Expression and clinical significance of metastasis-associated in colon cancer 1 （ MACC1 ） in HBV-related hepatocellular carcinoma

作　　者：常秀娟 王春平 曲建慧 陆荫英 陈艳 白文林 高旭东 郝莉燕 许桂林 王鋐 杨永平 

机构地区：[1]解放军第三О二医院肝脏肿瘤诊疗与研究中心,北京100039

出　　处：《中华肿瘤杂志》2012年第10期748-752,共5页Chinese Journal of Oncology

基　　金：国家科技重大专项基金（2008ZX100022018）;首都医学发展基金（2009-2041）

摘　　要：目的探讨结肠癌转移相关基因1（MACC1）mRNA在肝细胞癌中的表达及其临床意义。方法通过实时荧光定量PCR和免疫组化分别检测MACC1在354例肝癌组织中和10例正常肝组织的表达及其分布。采用单因素分析和Cox多因素回归分析MACC1mRNA表达的影响因素。结果MACC1mRNA在早期、中期和进展期肝癌组织中的中位相对表达量分别为0．00176、0．00249和0．00835，与正常对照组0．00059比较，差异均有统计学意义（均P〈0．01）。MACC1低表达组患者年龄、分期、瘤栓、分化程度与高表达组比较，差异均有统计学意义（均P〈0．05）。肝癌组织中MACC1表达的阳性率为42．4％（150／354），MACCl在正常肝细胞质呈阴性或弱阳性表达，部分患者肿瘤细胞核染色呈阳性。MACC1mRNA高表达者中位无瘤生存时间（34．0个月）和总生存时间（40．0个月）均低于低表达者（均为48．0个月，均P〈0．01）。Cox多因素回归分析结果显示，Child-Pugh分级、MACC1mRNA高表达是影响患者生存期和无瘤生存时间的独立影响因素。结论肝癌组织中MACClmRNA高表达与疾病进展和预后有关，可能成为肝癌术后复发、转移的潜在预测因子。Objective To clarify the expression and clinical significance of metastasis-associated in colon cancer 1 （MACC1） mRNA in hepatocellular carcinoma （HCC）. Methods The expression and distribution of MACC1 were assessed by quantitative real-time polymerase chain reaction （RT-PCR） and immunohistochemical staining （IHC） in a cohort of hepatitis B virus-related HCC, including 138 in early （A）, 96 in intermediate （B） and 120 in advanced stages （C）. The association of MACC1 mRNA with disease progression and outcomes was analyzed by univariate and multivariate Cox analysis. Results The intratumoral expressions of MACC1 mRNA in HCC stage Ⅰ （0.001 76, range ： 0.000 54-0.002 47）, stage Ⅱ （0.002 49, range ： 0.000 55-0.006 78 ） and stage m （0.008 35, range ： 0. 006 86-0. 009 88 ） were about 3-, 4- and lg-fold higher than that in the normal liver tissue （0. 000 59, range： 0. 000 57-0. 000 60）, respectively. Intratumoral expression of MACC1 mRNA increased with disease progression from stage I to stage m. HCC clinical staging classification, age, portal vein invasion and tumor differentiation were significantly associated with intratumoral high expression of MACC1 mRNA （ All P 〈 0. 05 ）. Immunohistochemical staining showed that there was an increased MACC1 expression in cytoplasm of HCC cells and positive nuclear staining in some cases. Increased MACC1 mRNA expression could predict poor outcome and recurrence in stage A and B HCC postoperatively. The median tumor-free survival and total survival of patients with high MACCI mRNA expression were 34.0 and 40 months, respectively, significantly lower than that in those with low expression （48.0 and 48.0 months） （ all P 〈 0.01 ）. Cox analysis showed that Child- Pugh grading and high expression of MACC1 mRNA were independent predictive factors, and high expression of MACC1 was an independent predictive factor affecting the tumor-free survival. Conclusions MACC1 mRNA up-regulation is a feature of disease progression

关 键 词：癌 肝细胞 结肠癌转移相关基因子1 复发 预后 

分 类 号：R735[医药卫生—肿瘤] R51[医药卫生—临床医学]