电针合穴、下合穴对慢性阻塞性肺病大鼠肠微循环及不同脏器血管活性肠肽的影响  

Effect of Electroacupuncture of “Chize”(LU 5) and “Shangjuxu”(ST 37) on Mesenteric Microcirculation and Vasoactive Intestinal Peptide Levels in the Lung,Colon and Hypothalamus in Rats with Chronic Obstructive Pulmonary Diseases

作　　者：杨伟宁[1,2] 李志同[2] 解秸萍[2] 李胜杰[2] 赵雅芳[2] 

机构地区：[1]西京医院中医科,西安710032 [2]北京中医药大学针灸推拿学院,北京100029

出　　处：《针刺研究》2012年第5期369-374,共6页Acupuncture Research

基　　金：北京中医药大学自主选题在读研究生资助项目(2011-JYBZZ-XS 099)

摘　　要：目的:观察电针合穴、下合穴对慢性阻塞性肺病(COPD)大鼠肠微循环及不同脏器血管活性肠肽(VIP)分布的影响,探讨"合主逆气而泄"与"合治内腑"作用特点与相关机制。方法:Wistar雄性大鼠随机分为正常组、模型组、尺泽组及上巨虚组,每组8只。除正常组外,余组均采用气管滴入脂多糖配合烟熏方法制备COPD模型。针刺组在造模的同时隔日针刺1次,共治疗12次。实验结束后用微循环显微测试仪观察各组大鼠肠系膜微循环的变化,放射免疫法测定肺、肠及下丘脑中VIP含量。结果:与正常组比较,模型组肠系膜微血管管径有增大趋势,与模型组相比,尺泽组与上巨虚组肠系膜微血管管径呈现缩小趋势,但差异均无统计学意义(P>0.05)。与正常组相比,模型组肠系膜微血管血流加快(P<0.05);与模型组相比,尺泽组、上巨虚组肠系膜微血管血流明显减慢(P<0.01)。各组肺、肠、下丘脑3种组织中,肺中VIP的含量最高(P<0.01);与正常组相比,模型组结肠中VIP含量明显升高(P<0.05);与模型组相比,尺泽组下丘脑中VIP含量降低(P<0.05)。结论:①肺病及肠,说明肺与大肠相表里的正确性;②"尺泽"与"上巨虚"均可使病理刺激引起的肠系膜微血管血流明显减慢,且"尺泽"可显著减少下丘脑中VIP含量,提示"合主逆气而泄"可能存在高位中枢的参与和调控。Objective To observe the influence of electroacupuncture （EA） of ＂Chize＂ （LU 5, He-sea acupoint） and ＂Shangjuxu＂（ST 37, lower He-sea acupoint） on mesenteric microcirculation, vasoactive intestinal peptide （VIP） levels in the lung, colon and hypothalamus tissues in rats with chronic obstructive pulmonary disease （COPD）, so as to investigate its mecha- nism underlying improvement of lung derived intestinal disorders in clinical practice. Methods Thirty-two male Wistar rats were randomly divided into normal control, model, EA-Ohize （LU 5, EA-LU 5） and EA-Shangjuxu （ST 37, EA-ST 37） groups, with 8 rats being in each group. COPD model was established by intratracheal infusion of Lipopolysaccharide （LPS, 1 mg/mL, 0.2 mL/ rat） and forced inhaling smoke, once daily for 28 days. EA was applied to bilateral LU 5 and ST 37 for 20 min, once every other day for 12 sessions. The state of mesenteric microcirculation was observed under microscope and divided into grade 0 （stagnation of blood flow）, Ⅰ （slow flowing and silt-like state）,Ⅱ （faster flowing with slight or obvious grainy feeling） and Ⅲ （fast flowing without grainy feeling）. The contents of VlP in the lung, colon and hypothalamus were detected using radioimmunoassay （RIA）. Resnlts Following modeling, the microvascular calibers were increased slightly in the model, EA-LU 5 and EA-ST 37 groups. Compared with the normal group, the blood flow velocity was increased significantly in model group （P〈0.05）. In comparison with the model group, the blood flow velocity was reduced significantly in EA-LU 5 and EA-ST 37 groups（P〈0.01）. There wereno significant differences between EA-LU 5 and EA-ST 37 groups in blood flow velocity, among the four groups in VIP contents of the lung tissue （P〉0.05）. The content of VIP in the colon was markedly higher in the model group than in the normal group, and that in the hypothalamus was obviously lower in the EA-LU 5 group than in the model group （all P〈0.05

关 键 词：慢性阻塞性肺病 电针干预 肠系膜微循环 血管活性肠肽肺 肠 下丘脑 

分 类 号：R224.2[医药卫生—中医基础理论]