重组腺病毒Ad-CRT/MAGE-A3联合表柔比星抑制乳腺癌细胞增殖及侵袭  

Inhibitory effect of recombinant adenovirus Ad-CRT/MAGE-A3 combined with epirubicin on proliferation and invasion of breast cancer cells

作　　者：刘新莉[1] 赵丹[1] 李晓曦[1] 李岩 马萍[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤研究所,辽宁沈阳110001

出　　处：《中国肿瘤生物治疗杂志》2012年第5期491-495,共5页Chinese Journal of Cancer Biotherapy

基　　金：辽宁省自然科学基金资助项目(No.20042073);辽宁省科技计划项目(No.2009225008-1)~~

摘　　要：目的:构建携带钙网蛋白(calreticulin,CRT)基因和黑素瘤抗原基因-A3(melanoma antigen gene-A3,MAGE-A3)的重组腺病毒载体Ad-CRT/MAGE-A3,观察其联合表柔比星对人乳腺癌细胞MDA-MB-231增殖及侵袭的抑制作用。方法:构建Ad-CRT/MAGE-A3载体,感染MDA-MB-231细胞后,荧光显微镜观察其最适感染复数(multiplicity of infection,MOI)。分表柔比星、Ad-GFP、Ad-CRT/MAGE-A3、表柔比星+Ad-GFP、表柔比星+Ad-CRT/MAGE-A3共5组,MTT法检测各组MDA-MB-231细胞的增殖,Transwell实验检测各组MDA-MB-231细胞的侵袭力。结果:成功构建重组腺病毒载体Ad-CRT/MAGE-A3,其感染MDA-MB-231细胞的最适MOI为100。与其他各组相比,应用Ad-CRT/MAGE-A3联合表柔比星能明显抑制MDA-MB-231细胞的增殖[(83.27±1.04)%vs(57.42±1.27)%,(43.26±0.95)%,(61.23±1.47)%,(55.38±1.62)%;P<0.05]和侵袭[(8.41±4.20)vs(14.62±5.33),(107.66±3.35),(100.60±4.42),(104.20±2.60);P<0.05]。结论:腺病毒载体Ad-CRT/MAGE-A3能增强表柔比星抑制乳腺癌细胞MDA-MB-231增殖及侵袭的能力。Objective ： To construct a recombinant adenovirus for calreticulin （CRT）/melanoma antigen gene-A3 （MAGE-A3） and evaluate its inhibitory effect on breast cancer MDA-MB-231 cells. Methods： Ad-CRT/MAGE-A3 vector was constructed and infected into MDA-MB-231 cells. The optimal multiplicity of infection （MOI） was obtained by fluorescence microscope method. There were five groups： epirubicin, Ad-GFP, Ad-CRT/MAGE-A3, epirubicin＋Ad-GFP and epirubicin＋Ad-CRT/MAGE-A3. The proliferation and invasion of MDA-MB-231 cells in different groups were detected by MTT and Transwell assays. Results： Recombinant adenovirus vector Ad-CRT/MAGE-A3 was constructed successfully, and its optimal MOI in MDA-MB-231 cells was 100. Ad-CRT/MAGE-A3 combined with epirubicin decreased the proliferation （／[83.27±1.04／]% vs ／[57.42±1.27／]%, ／[43.26±0.95／]%, ／[61.23±1.47／]%, ／[55.38±1.62／]%, P〈0.05） and the invasion （／[8.41±4.20／] vs ／[14.62±5.33／], ／[107.66±3.35／], ／[100.60±442／], ／[104.20±260／], P〈0.05） of MDA-MB-231 cells significantly. Conclusion： Recombinant adenovirus vector Ad-CRT/MAGE-A3 can enhance epirubicin to inhibit the proliferation and invasion of breast cancer MDA-MB-231 cells.

关 键 词：钙网蛋白基因 黑素瘤抗原基因-A3 重组腺病毒载体 表柔比星 乳腺癌 增殖 侵袭 

分 类 号：R737.9[医药卫生—肿瘤] R730.5[医药卫生—临床医学]