IL-21对慢性粒细胞白血病患者调节性T细胞的影响  

Effect of IL-21 on regulatory T cells in chronic myeloid leukemia patients

作　　者：王东萍[1] 张连生[1] 曾鹏云[1] 易良才[1] 

机构地区：[1]兰州大学第二医院血液科,甘肃兰州730030

出　　处：《中国肿瘤生物治疗杂志》2012年第5期517-520,共4页Chinese Journal of Cancer Biotherapy

基　　金：甘肃省科技支撑计划-社会发展计划(No.0804NKCA115)~~

摘　　要：目的:观察白细胞介素21(interleukin-21,IL-21)对慢性粒细胞白血病(chronic myeloid leukemia,CML)患者CD4+CD25+调节性T细胞(regulatory T cell,Treg)的作用,为CML的免疫治疗提供新思路。方法:收集兰州大学第二医院血液科初诊CML患者外周血单个核细胞(peripheral blood mononuclear cells,PBMCs),免疫磁珠法分选出CD4+T细胞。分别加入50、100、200 ng/ml IL-21与CD4+T细胞共培养72 h后,流式细胞术检测Treg的比例,并设生理盐水空白对照组。Real-time PCR法检测Treg细胞中Foxp3 mRNA的表达,ELISA法检测各组细胞上清液中IL-10、TGF-β的水平。结果:与空白对照组相比,50、100、200 ng/ml IL-21组CD4+CD25+Treg占CD4+T细胞的比例均降低[(3.42±0.76)%、(6.81±0.33)%、(7.98±0.76)%vs(12.09±0.91)%,P<0.05];且3组的Foxp3 mRNA均降低[(0.05±0.02)、(0.16±0.02)、(0.25±0.02)vs 1,P<0.05];Treg分泌IL-10量减少[(26.13±7.28)、(44.88±3.72)、(79.77±3.94)vs(133.00±12.32)pg/ml,P<0.05],分泌TGF-β量也减少[(9.25±0.84)、(16.70±1.00)、(20.47±1.60)vs(26.05±1.81)pg/ml,P<0.05]。结论:IL-21在体外可减少CML患者外周血中Treg的比例,并抑制其功能。Objective ： To obverse the effect of interleukin-21（IL-21） on CD4＋CD25＋ regulatory T cells （Tregs） in chronic myeloid leukemia （CML） patients. Methods： Peripheral blood mononuclear cells （PBMCs） were collected from newly diagnosed CML patients in the Hematology Department of Second Hospital of Lanzhou University, and CD4＋T cells were isolated by magnetic beads. CD4＋T cells were co-cultured with IL-21 （50, 100, and 200 ng/ml） and saline respectively for 72 h, and then the Treg ratio was determined by flow cytometry;  Foxp3 mRNA expression was examined by real-time PCR; and IL-10 and TGF-β in different cell supernatants were detected by ELISA. Results： Compared with the control group, the ratios of Treg in CD4＋T cells were decreased in 50 ng/ml, 100 ng/ml and 200 ng/ml IL-21 groups （[342±0.76]%, [6.81±0.33]%, [7.98±0.76]% vs [12.09±0.91]%, P〈0.05）; and  Foxp3 mRNA expressions were also decreased （[0.05±0.02], [0.16±002], [0.25±0.02] vs 1, P〈0.05）, the levels of IL-10 （[2613±7.28], [44.88±3.72], [79.77±394] vs [133.00±12.32] pg/ml, P〈0.05） and TGF-β （[9.25±0.84], [16.70±1.00], [20.47±1.59] vs [26.05±1.81] pg/ml, P〈0.05） in cell supernatants were significantly decreased. Conclusion： IL-21 can inhibit the ratio and function of Treg in peripheral blood of CML patients.

关 键 词：慢性粒细胞白血病(CML) IL-21 调节性T细胞(Treg) IL-10 TGF-β 

分 类 号：R733.7[医药卫生—肿瘤] R730.3[医药卫生—临床医学]