前动力蛋白2/前动力蛋白受体2基因杂合突变小鼠表型特征  

Phenotypes of PK2/PKR2 heterozygosis mutant mice

作　　者：肖玲[1] 周闻白[2] 周群勇[3] 胡仁明[2] 

机构地区：[1]复旦大学附属金山医院内分泌科,上海200540 [2]复旦大学附属华山医院内分泌科,上海200040 [3]美国加州大学欧文分校医学院药理部,美国加州92697

出　　处：《生殖医学杂志》2012年第5期465-471,共7页Journal of Reproductive Medicine

摘　　要：目的观察前动力蛋白(PK2)、前动力蛋白受体2(PKR2)基因单拷贝丢失后小鼠生长发育及生殖系统发育情况。方法对比观察PK2+/-、PKR2+/-、以及PK2+/-:PKR2+/-复合杂合突变小鼠与野生型小鼠体重增长、外生殖器发育情况;免疫荧光染色法观察四组小鼠颅内促性腺激素释放激素(GnRH)神经元分布;阴道冲洗细胞涂片评估法观察小鼠动情周期。结果三组杂合突变小鼠体重增长、外生殖器发育及颅内GnRH神经元分布与野生型小鼠相比均无显著差异,但杂合突变小鼠动情周期延长、无规律,双基因杂合突变小鼠表现最明显。结论 PK2、PKR2基因单拷贝丢失后虽不影响小鼠生殖系统和GnRH神经元发育,但雌鼠表现出动情周期紊乱。因此PK2/PKR2通路对生殖系统功能有调节作用。Objective. To observe the phenotype changes including weight growth and reproductive system development in PK2/PKR2 heterozygosis mutant mice. Methods. The weight gain and external genitalia development were compared between the three heterozygosis mutant mice groups and the wild type group. GnRH neurons distribution was observed by fluorescent staining method in four groups. The stage of estrus cycle was determined by cytologic evaluation of vaginal smears. The vaginal exfoliate ceils were smeared on glass slides, and the cytologic features were evaluated under microscopy Results： There were no changes in weight growth, external genitalia development and the GnRH neurons distribution in brain between the three mutant mice groups and the wild type mice group. The length of estrus cycle was extended in heterogeneous mice, especially in PKZ/PKR2 heterogeneous mutation mine. Conclusions. Loss of one copy of PK2 or PKR2 gene did not change reproductive system and GnRH neurons development except estrous cycle. So PK2/PKR2 pathway may play roles in the regulation of reproductive system function.

关 键 词：前动力蛋白2 前动力蛋白受体2 杂合突变 外生殖器 GnRH神经元 动情周期 

分 类 号：R[医药卫生]