补肾中药何首乌饮对衰老大鼠睾丸组织表皮生长因子及其受体表达的影响  

Effect of Heshouwuyin on the expression of EGF and EGFR in testes of aging rat

作　　者：牛嗣云[1] 周晓春[2] 高福禄[3] 丁良[1] 寇素茹[1] 刘晋芝[1] 

机构地区：[1]河北大学基础医学院,保定071000 [2]承德医学院基础研究所 [3]河北师范大学生命科学院,石家庄050024

出　　处：《解剖学杂志》2012年第5期588-591,共4页Chinese Journal of Anatomy

基　　金：河北大学人才引进基金（2010-183）;河北省卫生厅重点课题（20110151）

摘　　要：目的：观察何首乌饮对衰老雄性大鼠睾丸组织表皮生长因子（EGF）及其受体（EGFR）表达的影响。方法：选用D-半乳糖连续腹腔注射建立亚急性衰老大鼠模型，并用补肾中药何首乌饮进行干预，采用免疫组织化学和免疫印迹观察各实验组大鼠EGF、EGFR在睾丸组织表达的变化。结果：与正常对照组大鼠比较，模型组、自然恢复组大鼠睾丸组织EGF、EG—FR表达减少，而何首乌饮可以提高睾丸组织EGF、EGFR表达，与模型组和自然恢复组比较差异有统计学意义。结论：何首乌饮能够提高衰老大鼠睾丸组织EGF、EGFR表达，具有一定延缓睾丸组织衰老作用。Objective： To observe the effects of Heshouwuyin on the expression of EGF and EGFR in testes of aging male rat. Meth- ods： A subacute aging rats model was made by injecting D-gal into abdominal cavity continuously. At the same time, an intervention was performed with Heshouwuyirx Immunohistochemical method and Western blotting were used to detect the changes on the ex- pressions of EGF and EGFR in testes in the experimental groups. Results： Compared with the normal control group, the expressions of F_GF and EGFR in testes in the model and natural control group were significantly reduced. Compared with the model and natural control group, the expressions of EGF and EGFR in testes in the Heshouwuyin control group were significantly increased. Conclu- sion： Heshouwuyin can increase the expressions of EGF and EGFR, and have a certain anti-aging effect in testes.

关 键 词：何首乌饮 表皮生长因子 表皮生长因子受体 睾丸 衰老 大鼠 

分 类 号：R735.2[医药卫生—肿瘤]