福建畲族17个染色体短串联重复序列基因座遗传多态性  

Genetic polymorphisms of seventeen Y-chromosomal short tandem repeats loci in She nationality of Fujian province

作　　者：滕少康[1] 曹林枝[2] 林燕燕[2] 陈桐君[1] 郭月丽[2] 

机构地区：[1]福建漳州卫生职业学院人体解剖学教研室,漳州363000 [2]福建漳州卫生职业学院生物化学教研室,漳州363000

出　　处：《解剖学杂志》2012年第5期656-659,共4页Chinese Journal of Anatomy

基　　金：福建教育厅2010年科技计划项目（JA10300）;福建省漳州市2010年科技计划项目（Z2010092）

摘　　要：目的：调查Y染色体17个短串联重复序列（Y-STR）基因座的多态性及其单倍型在福建畲族人群的分布情况。方法：应用AmpF1STR@YfilerTM荧光标记复合扩增系统，对福建畲族152名无关男性个体血液样本进行17个Y-STR位点的复合扩增，应用ABIPRISM310遗传分析仪对扩增产物进行检测分析。结果：DYS456、DYS389I、DYS390、DYS389Ⅱ、DYS458、DYS19、DYS385a＼b、DYs393、DYS391、DYS439、DYS635、DYS392、y-GATA-H4、DYS437、DYS438、DYS448各位点遗传多样性（genediversity，GD值）分布在0．4196～0．9447之间。17个Y-STR位点共同构成的单倍型150种，其单倍型多样性为0．9998257。结论：福建畲族17个Y-STR位点具有丰富的遗传多样性，可为父权鉴定和父系进化研究提供有价值的遗传学资料。Objective： To investigate the Allelic and haplotype frequency distribution of seventeen short tandem repeat （STR） loci of Y chromosome in She nationality in Fujian province. Methods： Seventeen Y-STR loci, of which the template DNAs were extracted from blood samples of 152 unrelated male individuals in She population of Fujian province, were amplified by using the AmpFISTR YfilerTM. The PCR products were genotyped with ABI PRISM 310 genetic analyzer. Results： The Gene diversity ranged from 0. 419 6-0. 944 7 at DYS456, DYS389 1 , DYS390, DYS389 lI, DYS458, DYS19, DYS385a／b, DYS393, DYS391, DYSuI39, DYS635, DYS392, Y-GATA-H4, DYS437, DYS438, DYS448. A total of 150 different haplotypes were observed. The haplotype diversity value calculated from all 17 loci combined was 0. 999 825 7. Conclusion： The 17 Y-STR loci in She population of Fujian province are highly affluent genetic polymorphic and can offer valuable genetic data for paternity testing and paternal genetic lineages evolution.

关 键 词：Y染色体 短串联重复序列 畲族 遗传多态性 福建 

分 类 号：R394[医药卫生—医学遗传学]