Preparation of Red Component in Secondary Metabolites of Recombinant Hansenula anomala N6076 and Its GC-MS Detection  

作　　者：Yonghong FAN Xiang JIN Peihong MAO 

机构地区：[1]College of Life Science and Technology,Xinjiang University [2]Center of Ion Beam Biotechnology,College of Physics Science and Technology,Xinjiang University

出　　处：《Agricultural Biotechnology》2012年第4期58-60,共3页农业生物技术（英文版）

基　　金：Supported by National Natural Science Foundation of China (30960006);Natural Science Fund of Xinjiang University (BS080120);Postdoctoral Station of Geography from Xinjiang University

摘　　要：[ Objective] This study aimed to investigate the preparation of red component in secondary metabolites of recombinant Hansenula anomala strain N6076 and its GC-MS detection. [ Method] Thin-layer chromatography method was applied for large-scale preparation of red component in the secondary metabolites of re- combinant H. anomala strain N6076. The red component was dissolved in anhydrous ethanol for GC-MS detection and chemical structure comparison in the data- base to identify its type. [ Resultl The red component is preliminarily identified as a quinane compound, while no compound with exactly the same structure as the red component has been found in WILEY, Nist and Nbs compound libraries. [ Conclusion] This study laid the foundation for further NMR structure identification of the obtained red component and investigation of the relationship between its structure and biolo#cal effects.[ Objective] This study aimed to investigate the preparation of red component in secondary metabolites of recombinant Hansenula anomala strain N6076 and its GC-MS detection. [ Method] Thin-layer chromatography method was applied for large-scale preparation of red component in the secondary metabolites of re- combinant H. anomala strain N6076. The red component was dissolved in anhydrous ethanol for GC-MS detection and chemical structure comparison in the data- base to identify its type. [ Resultl The red component is preliminarily identified as a quinane compound, while no compound with exactly the same structure as the red component has been found in WILEY, Nist and Nbs compound libraries. [ Conclusion] This study laid the foundation for further NMR structure identification of the obtained red component and investigation of the relationship between its structure and biolo#cal effects.

关 键 词：Recombinant yeast Metabolitcs Red component Thin-layer chromatography GC-MS 

分 类 号：TS207.3[轻工技术与工程—食品科学] O657.63[轻工技术与工程—食品科学与工程]