淡豆豉药材的高效液相指纹图谱研究  

Study on HPLC fingerprint of Semen Sojae Preparatum(SSP)

作　　者：廖丽娜[1] 张明敏[1] 曹尉尉[2] 毛峻琴[3] 

机构地区：[1]安徽中医学院,安徽合肥230038 [2]解放军第411医院,上海200081 [3]解放军第85医院,上海200053

出　　处：《药学实践杂志》2012年第5期351-352,386,共3页Journal of Pharmaceutical Practice

基　　金：上海市卫生局项目(2010Y006A)

摘　　要：目的建立淡豆豉药材高效液相色谱指纹图谱(HPLC-FPS),为其质量控制提供依据。方法应用高效液相色谱法(HPLC),利用反相色谱柱:Diamonsil C18(250 mm×4.6 mm,5μm),流动相:乙腈-3%冰乙酸梯度洗脱,流速:1 ml/min,柱温:30℃,检测波长:261 nm,进样量:10μl。结果不同产地淡豆豉在化学成分方面具有明显相似性,可建立淡豆豉的指纹图谱。结论建立的HPLC指纹图谱可用于淡豆豉的质量评价。Objective To establish a HPLC fingerprint method for Semen Sojae Preparatumto quality control.Methods HPLC analysis was performed on a Diamonsil C18 column（250 mm×4.6 mm,5 μm）,the mobile phase was used in gradient elution consisted of methanol-water（3% acetic acid）,the flow rate was 1.0 ml /min,column temperature was 30℃ and UV detected was set at 261 nm,the sample weight：10 μl.Results The compound composition of different SSP origins was obviously comparable,which could be determined by HPLC fingerprint of the SSP.Conclusion This method could be utilized as a quality control measurement for SSP.

关 键 词：淡豆豉 高效液相色谱法 高效液相色谱指纹图谱 

分 类 号：R917[医药卫生—药物分析学] R284[医药卫生—药学]