CXCR4 RNA干扰序列筛选及对结肠癌细胞SW480生长的影响  

Influence of CXCR4 RNA interference for growth of colon carcinoma cell strain SW480

作　　者：王天宝[1] 戴强生[2] 尉秀清[3] 林维浩[1] 石汉平[1] 董文广[1] 

机构地区：[1]中山大学附属第一医院外科,广州510080 [2]中山大学附属第一医院肿瘤科,广州510080 [3]中山大学附属第三医院消化内科,广州510080

出　　处：《中华普通外科学文献（电子版）》2012年第5期19-22,共4页Chinese Archives of General Surgery(Electronic Edition)

基　　金：广东省科技计划资助项目(2010B060900100)

摘　　要：目的筛选细胞表面趋化因子受体(CXCR4) RNA干扰高效序列,探讨其对SW480细胞生长的影响。方法采用RT-PCR和Western blot方法选择最有效的CXCR4 RNA干扰序列,流式细胞术分析转染该序列后SW480细胞生长情况。结果 sihCXCR4-3序列可使CXCR4 RNA相对含量仅为SW480组的5.4%,CXCR4蛋白相对表达量为18.95%。sihCXCR4-3组细胞数[(0.92±0.06)×105]低于SW480细胞组[(1.38±0.04)×105,P=0.0050]及NCsihCXCR4组[(1.28±0.05)×105,P=0.0256];相对SW480组和NCsihCXCR4组,sihCXCR4-3组的抑制率分别为33.03%及28.00%。sihCXCR4-3组G1期百分率明显低于SW480细胞组[(59.5±0.57)%vs.(74.11±0.54)%,P=0.0167];S期百分率高于SW480组[(23.85±0.24)%vs.(14.62±0.46)%,P=0.0305]和NCsihRNA组[(17.63±0.48)%,P=0.0013]。结论 sihCXCR4-3序列可高效沉默CXCR4基因表达,抑制SW480细胞生长。Objective To select an effective CXCR4 RNA interference sequence and investigate its influence to the growth of SW480.Methods RT-PCR and Western blot were employed to find out the ideal RNA interference sequence,flow cytometry was used to analyze the growth and cell cycle of SW480.Results The relative expression of CXCR4 gene and protein in sihCXCR4-3 group was 5.4％ and 18.95％,resprectively.The mean of SW480 cell was lower in sihCXCR4-3 group [(0.92 ± 0.06) × 105]than in SW480 group[(1.38 ± 0.04) × 105,P=0.0050] and NCsihCXCR4 group[(1.28 ± 0.05) × 105,P =0.0256]. Comparing with SW480 and NCsihCXCR4 groups,the inhibion rate of sihCXCR4-3 group was 33.03％ and 28.00％, respectively. G2 stage percentage in sihCXCR4-3 group was obviously lower than in SW480 group[(59.5 ± 0.57)％ vs.(74.11 ± 0.54)％,P =0.0167],while S stage percentage was higher than in SW480 group [(23.85 ± 0.24)％ vs.(14.62 ± 0.46)％,P =0.0305] and in NCsihRNA group[(17.63 ± 0.48)％,P =0.0013].Conclusion sihCXCR4-3 inhibits effectively the expression of CXCR4 gene and cell proliferation of SW480.

关 键 词：CXCR4 RNA干扰 SW480 细胞周期 

分 类 号：R735.35[医药卫生—肿瘤]