年龄相关性黄斑变性肝肾亏虚证血浆蛋白组学研究  

Plasma Proteomic Analysis of Patients with Syndrome of Liver and Kidney Deficiency in Age-related Macular Degeneration

作　　者：徐新荣[1] 魏源华[2] 黄冰林[1] 仲路[1] 周欣[1] 王玲[3] 王富强[3] 

机构地区：[1]南京中医药大学附属医院眼科,江苏南京210029 [2]南京中医药大学附属医院检验科,江苏南京210029 [3]南京医科大学分析测试中心,江苏南京210029

出　　处：《南京中医药大学学报》2012年第5期443-447,共5页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省中医药领军人才培养工程资助项目(LJ200911);江苏省"六大人才高峰"第五批资助项目;江苏省中医药局2011-2012年度资助项目(LZ11041)

摘　　要：目的从蛋白质表达水平初步探讨年龄相关性黄斑变性(AMD)肝肾亏虚证的本质内涵。方法门诊确诊AMD肝肾亏虚证患者20例为观察组,AMD非肝肾亏虚证患者20例作为对照组。采集外周血,用双向凝胶电泳(2-DE)结合质谱分析、生物信息学分析方法对2组患者血浆蛋白进行比较蛋白质组学分析。结果发现有138个蛋白质斑点在2组凝胶中差异有统计学意义,获得有效鉴定及临床意义的蛋白质共16种。其中补体C1、补体因子B、成纤维细胞生长因子6、成纤维细胞生长因子13、肝细胞生长因子亚型6、胎盘生长因子亚型3、增殖细胞核抗原、激肽原1、真核翻译起始因子4、可溶性耐药相关钙结合蛋白亚型b、丝氨酸-苏氨酸蛋白激酶2表达上调,而补体因子I、色素上皮源性因子、αl-抗胰蛋白酶、αl-抗胰凝乳蛋白酶、NADH脱氢酶1α表达下调。结论 AMD肝肾亏虚证同时存在炎症损伤、补体激活、细胞增殖启动及细胞因子过表达,导致视网膜色素上皮细胞(RPE)损伤、代谢障碍及凋亡,同时也损伤血管内皮细胞,最终导致脉络膜新生血管(CNV)形成。这些差异蛋白可能作为AMD肝肾亏虚证的治疗靶点,对诊断和治疗有意义。OBJECTIVE To investigate the essence of TCM syndrome of liver and kidney deficiency（SLKD） in patient with age-related macular degeneration at the protein expression level.METHODS 20 patients with SLKD AMD and 20 without SLKD AMD were enrolled in the study.Plasma proteomic analyses of two groups were made using two-dimensional gel electrophoresis（2-DE）,mass spectrography and bioinformatics.RESULTS 138 differential expressed protein spots were detected between two groups.16 spots of them were successfully identified,of which,protein expressions of complement C1,B chain,isoform 1 of complement factor B,fibroblast growth factor 6（FGF 6）,FGF13,isoform 6 of hepatocyte growth factor,placental growth factor 3,proliferating cell nuclear antigen,kininogen-1,eukaryotic translation initiation factor 4,isoform 2 of serine/threonine-protein kinase（Akt2）,sorcin isoform b were up-regulated;protein expressions of light chain of factor I,pigment epithelium-derived factor,α1-antitrypsin,α1-antichymotrypsin,NADH dehydrogenase 1α were down-regulated.CONCLUSION Inflammatory injury,complement activation,cell proliferation promotion and cytokines over-expression could be involved in occurrence and development of SLKD AMD,which caused damage,metabolic disorders and apoptosis of retinal pigment epithelial cells.Moreover,they could impair vascular endothelial cells,finally induce choroidal neovascularization.The differential expressed protein could be regard as treatment targets;thus there is potential diagnostic and therapeutic significance of SLKD AMD.

关 键 词：年龄相关性黄斑变性 肝肾亏虚证 血浆蛋白 蛋白质组学 

分 类 号：R774.5[医药卫生—眼科]